Fish Arginase ELISA Kit | ARG elisa kit
Fish Arginase ELISA Kit
Reactivity
Fish
Synonyms
Arginase; Fish Arginase ELISA Kit; ARG elisa kit
Reactivity
Fish
Specificity
Pooled normal plasma from mouse, rat, pig, rabbit, sheep, canine, and guinea pig were assayed for cross-reactivity. No significant cross-reactivity was observed.
Samples
Human plasma, serum, urine, milk, saliva and cell culture samples
Sensitivity
0.0117 ng/mL.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for ARG elisa kit
Intended Uses: This human complement component 3 antigen assay is intended for the quantitative determination of total complement component 3 antigen in human plasma, serum, urine, milk, saliva and cell culture samples. For research use only.
Principle of the Assay: Human C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human C3 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human C3. Color development is proportional to the concentration of total C3 in the samples.
Background: Complement Component 3 (C3), the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation [lJ. C3 is proteolytically activated by C3-convertase to the anaphylatoxin C3a and the opsonizing agent C3b [2J. Serum concentrations of C3 are increased during acute and chronic inflammation such as rheumatoid arthritis, and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus [3J.
Principle of the Assay: Human C3 will bind to the capture antibody coated on the microtiter plate. After appropriate washing steps, biotin labeled anti-human C3 primary antibody binds to the captured protein. Excess primary antibody is washed away and bound antibody is reacted with peroxidase conjugated streptavidin. Following an additional washing step, TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human C3. Color development is proportional to the concentration of total C3 in the samples.
Background: Complement Component 3 (C3), the most abundant serum complement component, is a disulfide-linked 185kDa 1,637 amino acid glycoprotein which supports the classical, alternative, and lectin pathways of complement activation [lJ. C3 is proteolytically activated by C3-convertase to the anaphylatoxin C3a and the opsonizing agent C3b [2J. Serum concentrations of C3 are increased during acute and chronic inflammation such as rheumatoid arthritis, and are decreased due to increased consumption or autoimmune disorders such as systemic lupus erythematosus [3J.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
arginase
NCBI Official Symbol
Marky_0850
NCBI Protein Information
arginase
Protein Family
Similar Products
Product Notes
The Fish ARG (Catalog #AAA013551) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA013551 ELISA Kit recognizes Fish ARG. It is sometimes possible for the material contained within the vial of "Arginase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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