Goat Aquaporin 5 ELISA Kit | AQP-5 elisa kit

Goat Aquaporin 5 ELISA Kit

Cat. Num. AAA734214

• Gene Names: AQP5; PPKB; AQP-5
• Reactivity: Goat
• Synonyms: Aquaporin 5; Goat Aquaporin 5 ELISA Kit; AQP-5 elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This AQP-5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat AQP-5. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AQP-5 elisa kit

Principle of the assay: AQP-5 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for AQP-5. Standards or samples are then added to the microtiter plate wells and AQP-5 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AQP-5 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AQP-5 are added to each well to “sandwich” the AQP-5 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain AQP-5 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AQP-5 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AQP-5 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 4502183
• NCBI GeneID: 362
• NCBI Accession #: NP_001642.1
• NCBI GenBank Nucleotide #: NM_001651.3
• Molecular Weight: 28,292 Da
• NCBI Official Full Name: aquaporin-5
• NCBI Official Synonym Full Names: aquaporin 5
• NCBI Official Symbol: AQP5
• NCBI Official Synonym Symbols: PPKB; AQP-5
• NCBI Protein Information: aquaporin-5
• UniProt Protein Name: Aquaporin-5
• UniProt Gene Name: AQP5
• UniProt Synonym Gene Names: AQP-5
• UniProt Entry Name: AQP5_HUMAN

NCBI Description

Aquaporin 5 (AQP5) is a water channel protein. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions. AQP0, AQP2, AQP5, and AQP6 are closely related and all map to 12q13. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP5: Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Membrane protein, integral; Channel, misc.; Transporter, aquaporin family; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: microvillus; endoplasmic reticulum; integral to plasma membrane; apical plasma membrane; plasma membrane; basal plasma membrane

Molecular Function: protein binding; water channel activity; glycerol channel activity

Biological Process: odontogenesis; glycerol transport; camera-type eye morphogenesis; cellular water homeostasis; carbon dioxide transport; water transport; pancreatic juice secretion; excretion; transmembrane transport; saliva secretion