Typical Testing Data/Standard Curve (for reference only)
A ProlifeRation inducing ligand ELISA Kit | APRIL elisa kit
Rat A ProlifeRation inducing ligand ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: APIL ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-APIL antibody and an APIL-HRP conjugate. The assay sample and buffer are incubated together with APIL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APIL concentration since APIL from samples and APIL-HRP conjugate compete for the anti-APIL antibody binding site. Since the number of sites is limited, as more sites are occupied by APIL from the sample, fewer sites are left to bind APIL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APIL concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the tumor necrosis factor (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such as TNFRSF6/FAS and TNFRSF14/HVEM. Alternative splicing results in multiple transcript variants. Some transcripts that skip the last exon of the upstream gene (TNFSF12) and continue into the second exon of this gene have been identified; such read-through transcripts are contained in GeneID 407977, TNFSF12-TNFSF13. [provided by RefSeq, Oct 2010]
Uniprot Description
TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 17p13.1
Cellular Component: nucleoplasm; extracellular space; cytoplasm; cytosol; external side of plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; gene expression; signal transduction; positive regulation of isotype switching to IgA isotypes
Research Articles on APRIL
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Product Notes
The Rat APRIL tnfsf13 (Catalog #AAA735781) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA735781 ELISA Kit recognizes Rat APRIL. It is sometimes possible for the material contained within the vial of "A ProlifeRation inducing ligand, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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