APPL1 recombinant protein

Recombinant Human APPL1 protein

Cat. Num. AAA1561875

• Gene Names: APPL1; APPL; DIP13alpha
• Purity: >90% as determined by SDS-PAGE
• Synonyms: APPL1; Recombinant Human APPL1 protein; DCC-interacting protein 13-alpha; Dip13-alpha; dapter protein containing PH domain; PTB domain and leucine zipper motif 1; APPL1 recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human APPL1 (Phe404-Ser673) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for APPL1 recombinant protein

Multifunctional adapter protein that binds to various membrane receptors, nuclear factors and signaling proteins to regulate many processes, such as cell proliferation, immune response, endosomal trafficking and cell metabolism. Regulates signaling pathway leading to cell proliferation through interaction with RAB5A and subunits of the NuRD/MeCP1 complex. Functions as a positive regulator of innate immune response via activation of AKT1 signaling pathway by forming a complex with APPL1 and PIK3R1. Inhibits Fc-gamma receptor-mediated phagocytosis through PI3K/Akt signaling in macrophages. Regulates TLR4 signaling in activated macrophages. Involved in trafficking of the TGFBR1 from the endosomes to the nucleus via microtubules in a TRAF6-dependent manner. Plays a role in cell metabolism by regulating adiponecting and insulin signaling pathways. Required for fibroblast migration through HGF cell signaling. Positive regulator of beta-catenin/TCF-dependent transcription through direct interaction with RUVBL2/reptin resulting in the relief of RUVBL2-mediated repression of beta-catenin/TCF target genes by modulating the interactions within the beta-catenin-reptin-HDAC complex

Product Categories/Family for APPL1 recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 6912242
• NCBI GeneID: 26060
• NCBI Accession #: NP_036228.1
• NCBI GenBank Nucleotide #: NM_012096.2
• UniProt Accession #: Q9UKG1; Q9P2B9
• Molecular Weight: 79,663 Da
• NCBI Official Full Name: DCC-interacting protein 13-alpha
• NCBI Official Synonym Full Names: adaptor protein, phosphotyrosine interaction, PH domain and leucine zipper containing 1
• NCBI Official Symbol: APPL1
• NCBI Official Synonym Symbols: APPL; DIP13alpha
• NCBI Protein Information: DCC-interacting protein 13-alpha; dip13-alpha; AKT2 interactor; signaling adaptor protein DIP13alpha; adapter protein containing PH domain, PTB domain and leucine zipper motif 1; adaptor protein containing pH domain, PTB domain and leucine zipper motif 1
• UniProt Protein Name: DCC-interacting protein 13-alpha
• Protein Family: DCC-interacting protein
• UniProt Gene Name: APPL1
• UniProt Synonym Gene Names: APPL; DIP13A; KIAA1428; Dip13-alpha
• UniProt Entry Name: DP13A_HUMAN

NCBI Description

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

APPL: an effector protein of the small GTPase Rab5, a key regulator of endocytosis. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Contains a PH domain and an N-terminal PID domain. Its interaction with RAB5A/Rab5 is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Phosphorylated upon DNA damage.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: early endosome membrane; cytoplasm; endosome membrane; vesicle membrane; NuRD complex; nucleus; cytosol

Molecular Function: protein kinase B binding; identical protein binding; protein binding

Biological Process: cell proliferation; positive regulation of apoptosis; insulin receptor signaling pathway; regulation of glucose import; cell cycle; signal transduction

Disease: Maturity-onset Diabetes Of The Young, Type 14