DCC-interacting Protein 13-alpha Recombinant Protein | APPL1 recombinant protein

DCC-interacting Protein 13-alpha

Cat. Num. AAA398075

• Gene Names: APPL1; APPL; MODY14; DIP13alpha
• Applications: ELISA, Western Blot
• Purity: >90%; SDS PAGE to determine purity of the protein
• Synonyms: DCC-interacting Protein 13-alpha; APPL; DIP13alpha; Recombinant APPL1 with His-tag (Human); APPL1 recombinant protein

• Host: E Coli
• Purity/Purification: >90%; SDS PAGE to determine purity of the protein
• Form/Format: Lyophilized in 1mg/mL in PBS
• Sequence: MSYYHHHHHH DYDIPTTENL YFQGAMGSGI QPGIDKLPIE ETLEDSPQTRSLLGVFEEDA TAISNYMNQL YQAMHRIYDA QNELSAATHL TSKLLKEYEKQRFPLGGDDE VMSSTLQQFSKVIDELSSCH AVLSTQLADA MMFPITQFKERDLKEILTLK EVFQIASNDH DAAINRYSRL SKKRENDKVK YEVTEDVYTSRKKQHQTMMH YFCALNTLQY KKKIALLEPL LGYMQAQISF FKMGSENLNE QLEEFLANIG TSVQNVRREM DSDIETMQQT IEDLEVASDP LYVPDPDPTK FPVNRNLTRK AGYLNARNKT GLVSSTWDRQ FYFTQGGNLM SQARGDVAGG LAMDIDNCSV MAVDCEDRRY CFQITSFDGK KSSILQAESK KDHEEWICTI NNISKQIYLS ENPEETAARV NQSALEAVTP SPSFQQRHES LRPAAGQSRP PTARTSSSGS LGSESTNLAA LSLDSLVAPD TPIQFDIISP VCEDQPGQAK AFGQGGRRTN PFGESGGSTK SETEDSILHQ LFIVRFLGSM EVKSDDHPDV VYETMRQILA ARAIHNIFRM TESHLLVTCD CLKLIDPQTQ VTRLTFPLPC VVLYATHQEN KRLFGFVLRT SSGRSESNLS SVCYIFESNN EGEKICDSVG LAKQIALHAE LDRRASEKQK EIERVKEKQQ KELNKQKQIE KDLEEQSRLI AASSRPNQAS SEGQFVVLSS SQSEESDLGE GGKKRESEA
• Sequence Length: 709

• Applicable Applications for APPL1 recombinant protein: ELISA (EIA), Western Blot (WB)
• Species: Human
• Tag: His Tag
• Reconstitution: Add deionized water to prepare a working stock solution of approximately 1mg/mL and let the lyophilized pellet dissolve completely.
• Quality Control Test: BCA to determine quantity of the protein.
• Preparation and Storage: Store lyophilized protein at -20 degree C. Aliquot reconstituted protein and store at -80 degree C. Avoid repeated freezing /thawing cycles.

Related Product Information for APPL1 recombinant protein

APPL1, an adaptor protein containing an NH2-terminalBin/Amphiphiphysin/Rvs (BAR) domain, a central pleckstrin homology (PH) domain and a COOH-terminal phosphotyrosine binding (PTB) domain [1], was originally identified as an interacting partner of Akt in a yeast two-hybrid assay using Akt2 as a bait [2]. APPL1 binds to a number of cell surface receptors (TrkA[3, 4], DCC[5], adiponectin [6, 7], FSH[8]) and intracellular signaling molecules (small GTPase Rab5[9], GIPC[4] and inositol 5-phosphatase[10], suggesting that APPL1 may act as a common relay to coordinate diverse signaling cascades. APPL1 potentiates insulin-mediated Akt activation by counteracting the effect of the Akt inhibitor TRB3 [11].
Total 739AA, Mw: 83.2 kDa (calculated). N-terminal His-tag and TEV cleavage site, 31 extra AA.

NCBI and Uniprot Product Information

• NCBI GI #: 6912242
• NCBI GeneID: 26060
• NCBI GenBank Nucleotide #: NP_036228.1
• UniProt Accession #: Q9UKG1
• Molecular Weight: 83.2kDa
• NCBI Official Full Name: DCC-interacting protein 13-alpha
• NCBI Official Synonym Full Names: adaptor protein, phosphotyrosine interacting with PH domain and leucine zipper 1
• NCBI Official Symbol: APPL1
• NCBI Official Synonym Symbols: APPL; MODY14; DIP13alpha
• NCBI Protein Information: DCC-interacting protein 13-alpha
• UniProt Protein Name: DCC-interacting protein 13-alpha
• Protein Family: DCC-interacting protein
• UniProt Gene Name: APPL1
• UniProt Synonym Gene Names: APPL; DIP13A; KIAA1428; Dip13-alpha
• UniProt Entry Name: DP13A_HUMAN

NCBI Description

The protein encoded by this gene has been shown to be involved in the regulation of cell proliferation, and in the crosstalk between the adiponectin signalling and insulin signalling pathways. The encoded protein binds many other proteins, including RAB5A, DCC, AKT2, PIK3CA, adiponectin receptors, and proteins of the NuRD/MeCP1 complex. This protein is found associated with endosomal membranes, but can be released by EGF and translocated to the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

APPL: an effector protein of the small GTPase Rab5, a key regulator of endocytosis. Required for the regulation of cell proliferation in response to extracellular signals from an early endosomal compartment. Links Rab5 to nuclear signal transduction. Contains a PH domain and an N-terminal PID domain. Its interaction with RAB5A/Rab5 is essential for its recruitment to endosomal membranes as well as its role in cell proliferation. Binds DCC and the catalytic domain of the inactive form of AKT2 through its PID domain. Binds PIK3CA and subunits of the NuRD/MeCP1 complex. Phosphorylated upon DNA damage.

Protein type: Adaptor/scaffold; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 3p14.3

Cellular Component: early endosome membrane; cytoplasm; endosome membrane; vesicle membrane; NuRD complex; nucleus; cytosol

Molecular Function: protein kinase B binding; identical protein binding; protein binding

Biological Process: cell proliferation; positive regulation of apoptosis; insulin receptor signaling pathway; regulation of glucose import; cell cycle; signal transduction

Disease: Maturity-onset Diabetes Of The Young, Type 14