APOM recombinant protein

Recombinant Human APOM Protein (Fc tag)

Cat. Num. AAA2545304

• Gene Names: APOM; G3a; NG20; apo-M; HSPC336
• Purity: > 85% as determined by reducing SDS-PAGE.
• Synonyms: APOM; Recombinant Human APOM Protein (Fc tag); apo-M; DADB-127H9.5; G3a; HSPC336; NG20; APOM recombinant protein

• Host: HEK293 Cells
• Purity/Purification: > 85% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% Mannitol, 0.01% Tween-80.
• Sequence Length: 116

• Species: Human
• Sequence: Met 1-Asn 188
• Tag: C-Fc
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Reconstitution: It is recommended that sterile water(200ul) be added to the vial to prepare a stock solution of 0.25mg/mL. Concentration is measured by UV-Vis.
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; ; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-PAGE

Related Product Information for APOM recombinant protein

ApoM (apolipoprotein M) is an apolipoprotein and member of the lipocalin protein family. The lipocalins share limited regions of sequence homology and a common tertiary structure architecture. They have an eight-stranded, antiparallel, symmetrical _-barrel fold, which is in essence a beta sheet which has been rolled into a cylindrical shape. Inside this barrel is located a ligand binding site. They transport small hydrophobic molecules such as steroids, bilins, retinoids, and lipids. Lipocalins have been associated with many biological processes, among them immune response, pheromone transport, biological prostaglandin synthesis, retinoid binding, and cancer cell interactions. Lipocalins are comparatively small in size, and are thus less complicated to study as opposed to large, bulky proteins. They can also bind to various ligands for different biological purposes. ApoM is associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. ApoM is involved in lipid transport and can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.

NCBI and Uniprot Product Information

• NCBI GI #: 371873523
• NCBI GeneID: 55937
• NCBI Accession #: NP_001243098.1
• NCBI GenBank Nucleotide #: NM_001256169.1
• UniProt Accession #: O95445; Q5SRP4; Q9P046; Q9UMP6; B0UX98
• Molecular Weight: Molecular Mass: 45.6 kDa; Actual Protein Molecular Mass: 50 kDa
• NCBI Official Full Name: apolipoprotein M isoform 2
• NCBI Official Synonym Full Names: apolipoprotein M
• NCBI Official Symbol: APOM
• NCBI Official Synonym Symbols: G3a; NG20; apo-M; HSPC336
• NCBI Protein Information: apolipoprotein M
• UniProt Protein Name: Apolipoprotein M
• Protein Family: Apomucin
• UniProt Gene Name: APOM
• UniProt Synonym Gene Names: G3A; NG20; Apo-M; ApoM

NCBI Description

The protein encoded by this gene is an apolipoprotein and member of the lipocalin protein family. It is found associated with high density lipoproteins and to a lesser extent with low density lipoproteins and triglyceride-rich lipoproteins. The encoded protein is secreted through the plasma membrane but remains membrane-bound, where it is involved in lipid transport. Alternate splicing results in both coding and non-coding variants of this gene. [provided by RefSeq, Jan 2012]

Uniprot Description

APOM: Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid. Plasma protein. Expressed in liver and kidney. Belongs to the calycin superfamily. Lipocalin family. Highly divergent.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.33

Cellular Component: extracellular region; integral to plasma membrane

Molecular Function: antioxidant activity; lipid transporter activity; phospholipid binding

Biological Process: cholesterol efflux; cholesterol homeostasis; retinoid metabolic process; reverse cholesterol transport