Apolipoprotein H Recombinant Protein | APOH recombinant protein

Recombinant Apolipoprotein H (APOH)

Cat. Num. AAA2030892

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Apolipoprotein H; Recombinant Apolipoprotein H (APOH); APOH recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 8.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- G RRMWPINTLK CTPRVCPFAG ILENGVVRYT TFEYPNTIGF ACNPGYYLNG TSSSKCTEEG KWSPELPVCA RITCPPPPIP KFAALKEYKT SVGNSSFYQD TVVFKCLPHF AMFGNDTVTC TAHGNWTQLP ECREVKCPFP SRPDNGFVNY PAKPVLSYKD KAVFGCHETY KLDGPEEVEC TKTGNWSALP SCKASCKLSV KKATVLYQGQ RVKIQDQFKN GMMHGDKVHF YCKNKEKKCS YTEEAQCIDG TIEIPKCFKEHSSLAFWKTD ASDVTPC
• Sequence Length: 345

• Applicable Applications for APOH recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 35.0kDa
• Accurate Molecular Mass (KD): 38kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal Tags, His-tag and T7-tag
• Organism Species: Rattus norvegicus (Rat)
• Fragment: Gln25~Leu145
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 178857
• Molecular Weight: 38,298 Da
• NCBI Official Full Name: apolipoprotein H
• UniProt Protein Name: Beta-2-glycoprotein 1
• Protein Family: Beta-2-glycoprotein
• UniProt Gene Name: APOH
• UniProt Synonym Gene Names: B2G1; Apo-H; B2GPI; Beta(2)GPI
• UniProt Entry Name: APOH_HUMAN

Uniprot Description

APOH: Binds to various kinds of negatively charged substances such as heparin, phospholipids, and dextran sulfate. May prevent activation of the intrinsic blood coagulation cascade by binding to phospholipids on the surface of damaged cells.

Protein type: Secreted, signal peptide; Secreted; Lipid-binding

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface

Molecular Function: heparin binding; identical protein binding; protein binding; phospholipid binding; glycoprotein binding; lipid binding

Biological Process: negative regulation of angiogenesis; negative regulation of myeloid cell apoptosis; positive regulation of blood coagulation; negative regulation of fibrinolysis; triacylglycerol metabolic process; negative regulation of endothelial cell proliferation; positive regulation of lipoprotein lipase activity; negative regulation of blood coagulation; triacylglycerol transport; blood coagulation, intrinsic pathway; plasminogen activation; regulation of fibrinolysis