Typical Testing Data/Standard Curve (for reference only)
Rabbit Apolipoprotein C3 ELISA Kit | APOC3 elisa kit
Rabbit Apolipoprotein C3 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: APoC3 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-APoC3 antibody and an APoC3-HRP conjugate. The assay sample and buffer are incubated together with APoC3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APoC3 concentration since APoC3 from samples and APoC3-HRP conjugate compete for the anti-APoC3 antibody binding site. Since the number of sites is limited, as more sites are occupied by APoC3 from the sample, fewer sites are left to bind APoC3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APoC3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]
Uniprot Description
APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family.
Protein type: Secreted; Secreted, signal peptide; Lipid-binding
Chromosomal Location of Human Ortholog: 11q23.3
Cellular Component: extracellular space; chylomicron; early endosome; extracellular region
Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity
Biological Process: response to drug; phototransduction, visible light; response to peptide hormone stimulus; cholesterol metabolic process; regulation of Cdc42 protein signal transduction; cholesterol efflux; lipoprotein metabolic process; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; negative regulation of lipid catabolic process; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; inflammatory response; negative regulation of lipid metabolic process; retinoid metabolic process; triacylglycerol mobilization; response to nutrient; negative regulation of fatty acid biosynthetic process
Disease: Apolipoprotein C-iii Deficiency
Research Articles on APOC3
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Product Notes
The Rabbit APOC3 apoc3 (Catalog #AAA728041) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728041 ELISA Kit recognizes Rabbit APOC3. It is sometimes possible for the material contained within the vial of "Apolipoprotein C3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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