Human Apolipoprotein C3 ELISA Kit | APOC3 elisa kit

Human Apolipoprotein C3 ELISA Kit

Cat. Num. AAA721468

• Gene Names: APOC3; HALP2; APOCIII
• Reactivity: Human
• Synonyms: Apolipoprotein C3; Human Apolipoprotein C3 ELISA Kit; APOC3 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This Apo-C3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human Apo-C3. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOC3 elisa kit

Principle of the assay: Apo-C3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-Apo-C3 antibody and an Apo-C3-HRP conjugate. The assay sample and buffer are incubated together with Apo-C3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the Apo-C3 concentration since Apo-C3 from samples and Apo-C3-HRP conjugate compete for the anti-Apo-C3 antibody binding site. Since the number of sites is limited, as more sites are occupied by Apo-C3 from the sample, fewer sites are left to bind Apo-C3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The Apo-C3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOC3 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4557323
• NCBI GeneID: 345
• NCBI Accession #: NP_000031.1
• NCBI GenBank Nucleotide #: NM_000040.1
• Molecular Weight: 10,852 Da
• NCBI Official Full Name: apolipoprotein C-III
• NCBI Official Synonym Full Names: apolipoprotein C-III
• NCBI Official Symbol: APOC3
• NCBI Official Synonym Symbols: HALP2; APOCIII
• NCBI Protein Information: apolipoprotein C-III; apo-CIII; apoC-III; apolipoprotein C3
• UniProt Protein Name: Apolipoprotein C-III
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC3
• UniProt Synonym Gene Names: Apo-CIII; ApoC-III
• UniProt Entry Name: APOC3_HUMAN

NCBI Description

Apolipoprotein C-III is a very low density lipoprotein (VLDL) protein. APOC3 inhibits lipoprotein lipase and hepatic lipase; it is thought to delay catabolism of triglyceride-rich particles. The APOA1, APOC3 and APOA4 genes are closely linked in both rat and human genomes. The A-I and A-IV genes are transcribed from the same strand, while the A-1 and C-III genes are convergently transcribed. An increase in apoC-III levels induces the development of hypertriglyceridemia. [provided by RefSeq, Jul 2008]

Uniprot Description

APOC3: Inhibits lipoprotein lipase and hepatic lipase and decreases the uptake of lymph chylomicrons by hepatic cells. This suggests that it delays the catabolism of triglyceride-rich particles. Defects in APOC3 are the cause of hyperalphalipoproteinemia type 2 (HALP2). HALP2 is a condition characterized by high levels of high density lipoprotein (HDL) and increased HDL cholesterol levels. Belongs to the apolipoprotein C3 family.

Protein type: Secreted; Lipid-binding; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: extracellular space; chylomicron; early endosome; extracellular region

Molecular Function: lipase inhibitor activity; cholesterol binding; phospholipid binding; enzyme regulator activity

Biological Process: response to drug; response to peptide hormone stimulus; cholesterol metabolic process; phototransduction, visible light; regulation of Cdc42 protein signal transduction; lipoprotein metabolic process; cholesterol efflux; G-protein coupled receptor protein signaling pathway; cholesterol homeostasis; triacylglycerol metabolic process; reverse cholesterol transport; phospholipid efflux; negative regulation of lipid catabolic process; lipoprotein transport; triacylglycerol catabolic process; negative regulation of lipoprotein lipase activity; negative regulation of receptor-mediated endocytosis; negative regulation of lipid metabolic process; retinoid metabolic process; triacylglycerol mobilization; inflammatory response; response to nutrient; negative regulation of fatty acid biosynthetic process

Disease: Apolipoprotein C-iii Deficiency