Human Apolipoprotein A2 ELISA Kit | APOA2 elisa kit

Human Apolipoprotein A2 ELISA Kit

Cat. Num. AAA720600

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Reactivity: Human
• Synonyms: Apolipoprotein A2; Human Apolipoprotein A2 ELISA Kit; APOA2 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive or Sandwich
• Detection Range: 100-2500ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ApoA2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ApoA2. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA2 elisa kit

Principle of the assay: ApoA2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti- ApoA2 antibody and an ApoA2-HRP conjugate.The assay sample and buffer are incubated together with ApoA2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ApoA2 concentration since ApoA2 from samples and ApoA2-HRP conjugate compete for the anti-ApoA2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ApoA2 from the sample, fewer sites are left to bind ApoA2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ApoA2 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for APOA2 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 4502149
• NCBI GeneID: 336
• NCBI Accession #: NP_001634.1
• NCBI GenBank Nucleotide #: NM_001643.1
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-II
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II; apolipoprotein A2
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II; ProapoA-II
• UniProt Entry Name: APOA2_HUMAN

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA2: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Belongs to the apolipoprotein A2 family.

Protein type: Lipid-binding; Endoplasmic reticulum; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q23.3

Cellular Component: chylomicron; endoplasmic reticulum lumen; early endosome; extracellular region; cytosol

Molecular Function: lipid transporter activity; protein binding; protein homodimerization activity; lipase inhibitor activity; protein heterodimerization activity; cholesterol transporter activity; cholesterol binding; phospholipid binding; phosphatidylcholine binding; lipid binding; high-density lipoprotein binding; apolipoprotein receptor binding

Biological Process: positive regulation of catalytic activity; phototransduction, visible light; protein folding; viral reproduction; negative regulation of cholesterol transport; response to glucocorticoid stimulus; regulation of protein stability; diacylglycerol catabolic process; positive regulation of lipid catabolic process; cellular lipid metabolic process; negative regulation of lipase activity; negative regulation of lipid catabolic process; regulation of cholesterol absorption; phospholipid efflux; response to glucose stimulus; retinoid metabolic process; response to drug; cholesterol metabolic process; organ regeneration; protein amino acid oxidation; phospholipid catabolic process; positive regulation of interleukin-8 biosynthetic process; lipoprotein metabolic process; cholesterol efflux; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; reverse cholesterol transport; triacylglycerol metabolic process; response to estrogen stimulus; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; acute inflammatory response

Disease: Hypercholesterolemia, Familial