Human apolipoprotein A-II ELISA Kit | APOA2 elisa kit

Human Apolipoprotein A2, apo-A2 ELISA Kit

Cat. Num. AAA704270

• Gene Names: APOA2; apoAII; Apo-AII; ApoA-II
• Reactivity: Human
• Synonyms: apolipoprotein A-II; Human Apolipoprotein A2; apo-A2 ELISA Kit; APOA2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human APOA2. No significant cross-reactivity or interference between Human APOA2 and analogues was observed.
• Sequence Length: 100

• Detection Range: 6.2 ug/ml-400 ug/ml?
• Sensitivity: 1.6 ug/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Immunity
• Protein Biological Process 3: Host-virus interaction
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for APOA2 elisa kit

Introduction: Apolipoprotein A2 is a 17.5 kDa protein with a pI of 4.9. Lipids, as such areinsoluble in plasma. They combine with apolipoproteins to form lipoproteinsand this process solubilises lipids in plasma, providing a transport systemfor lipids. Apolipoproteins also play an important role in lipoprotein receptorrecognition and regulation of certain enzymes in lipoprotein metabolism.Two main forms of high density lipoprotein exist; both contain APOA1, butone type only contains APO A2.For more information on APO A2 pleasedownload the PDF catalogue below.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with anantibody specific to apo-A2. Standards or samples are then added to theappropriate microtiter plate wells with a biotin-conjugated polyclonalantibody preparation specific for apo-A2 and Avidin conjugated toHorseradish Peroxidase (HRP) is added to each microplate well andincubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solutionis added to each well. Only those wells that contain apo-A2,biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit achange in color. The enzyme-substrate reaction is terminated by theaddition of a sulphuric acid solution and the color change is measuredspectrophotometrically at a wavelength of 450 nm +/- 2 nm. Theconcentration of apo-A2 in the samples is then determined by comparingthe O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502149
• NCBI GeneID: 336
• NCBI Accession #: NP_001634.1
• NCBI GenBank Nucleotide #: NM_001643.1
• UniProt Accession #: P02652; B2R524
• Molecular Weight: 11,175 Da
• NCBI Official Full Name: apolipoprotein A-II preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-II
• NCBI Official Symbol: APOA2
• NCBI Official Synonym Symbols: apoAII; Apo-AII; ApoA-II
• NCBI Protein Information: apolipoprotein A-II; apolipoprotein A2
• UniProt Protein Name: Apolipoprotein A-II
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA2
• UniProt Synonym Gene Names: Apo-AII; ApoA-II; ProapoA-II
• UniProt Entry Name: APOA2_HUMAN

NCBI Description

This gene encodes apolipoprotein (apo-) A-II, which is the second most abundant protein of the high density lipoprotein particles. The protein is found in plasma as a monomer, homodimer, or heterodimer with apolipoprotein D. Defects in this gene may result in apolipoprotein A-II deficiency or hypercholesterolemia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism.