Apelin (APLN) Recombinant Protein | APLN recombinant protein

Recombinant Apelin (APLN)

Cat. Num. AAA2010771

• Gene Names: Apln; Apel
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Apelin (APLN); Recombinant Apelin (APLN); APLN recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.; MHHHHHHS SGLVPRGSGM KETAAAKFER QHMDSPDLGT DDDDKAMADI GSEF-AVCGVPLMLP PDGKGLEEGN MRYLVKPRTS RTGPGAWQGG RRKFRRQRPR LSHKGPMPF
• Sequence Length: 77

• Applicable Applications for APLN recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Ala19~Phe77 (Accession # Q9R0R3) with two N-terminal Tags, His-tag and S-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

NCBI and Uniprot Product Information

• NCBI GI #: 13928836
• NCBI GeneID: 58812
• NCBI Accession #: NP_113800.1
• NCBI GenBank Nucleotide #: NM_031612.3
• UniProt Accession #: Q9R0R3
• Molecular Weight: 12.3kDa
• NCBI Official Full Name: apelin preproprotein
• NCBI Official Synonym Full Names: apelin
• NCBI Official Symbol: Apln
• NCBI Official Synonym Symbols: Apel
• NCBI Protein Information: apelin; APJ endogenous ligand; apelin, AGTRL1 ligand
• UniProt Protein Name: Apelin
• Protein Family: Apelin
• UniProt Gene Name: Apln
• UniProt Synonym Gene Names: Apel
• UniProt Entry Name: APEL_RAT

Uniprot Description

Function: Endogenous ligand for APJ. Apelin-36 dissociates more hardly than (pyroglu)apelin-13 from APJ. The oral intake in the colostrum and the milk could have a role in the modulation of the immune responses in neonates. May also have a role in the central control of body fluid homeostasis by influencing AVP release and drinking behavior. Ref.5

Subcellular location: Secreted.

Tissue specificity: Widely expressed in the adult, with highest levels in the mammary gland of lactating rats, very high levels in the lung, intermediate levels in the spinal cord, ovary, adipose tissue, brain (neuronal cell bodies and fibers in the supraoptic and the paraventricular nuclei), heart and testis, and lowest levels in the pituitary gland, kidney, stomach, uterus and pancreas. The lung, testis and uterus mainly contain a large form that looks like apelin-36, whereas the mammary gland seems to contain 2 forms of apelin, a large form close to apelin-36 and a small form close to apelin-13.

Developmental stage: Highly expressed in neonatal tissues. In the adult, reaches a maximal level around parturition.

Induction: During pregnancy and lactation.

Post-translational modification: Several active peptides may be produced by proteolytic processing of the precursor.

Sequence similarities: Belongs to the apelin family.