Bovine Activated Protein C ELISA Kit | APC elisa kit

Bovine Activated Protein C ELISA Kit

Cat. Num. AAA43637

• Gene Names: APC; GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• Reactivity: Bovine
• Synonyms: Activated Protein C; Bovine Activated Protein C ELISA Kit; APC elisa kit

• Reactivity: Bovine
• Specificity: No significant cross-reactivity or interference between Bovine APC and analogues was observed.
• Sequence Length: 2843

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 62.5 ng/ml – 2000 ng/ml.
• Sensitivity: 10 ng/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated APC concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine APC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APC and APC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for APC elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated APC concentrations in Bovine serum, plasma and other body fluids. Using Purified Bovine APC antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add APC and APC antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of APC in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 53759122
• NCBI GeneID: 324
• NCBI Accession #: NP_000029.2
• NCBI GenBank Nucleotide #: NM_000038.5
• UniProt Accession #: P25054; Q15162; Q15163; Q93042; D3DT03
• Molecular Weight: 311,646 Da
• NCBI Official Full Name: adenomatous polyposis coli protein isoform b
• NCBI Official Synonym Full Names: adenomatous polyposis coli
• NCBI Official Symbol: APC
• NCBI Official Synonym Symbols: GS; DP2; DP3; BTPS2; DP2.5; PPP1R46
• NCBI Protein Information: adenomatous polyposis coli protein; deleted in polyposis 2.5; adenomatosis polyposis coli tumor suppressor; protein phosphatase 1, regulatory subunit 46
• UniProt Protein Name: Adenomatous polyposis coli protein
• Protein Family: Adenomatous polyposis coli
• UniProt Gene Name: APC
• UniProt Synonym Gene Names: DP2.5; Protein APC
• UniProt Entry Name: APC_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq, Jul 2008]

Uniprot Description

APC: a tumor suppressor. Defects in APC are a cause of familial adenomatous polyposis (FAP). Promotes rapid degradation of CTNNB1 and participates in Wnt signaling. Activity is correlated with its phosphorylation state. Two splice-variant isoforms have been described.

Protein type: Tumor suppressor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 5q21-q22

Cellular Component: centrosome; tight junction; beta-catenin destruction complex; cytosol; nucleoplasm; kinetochore; cell-cell adherens junction; lamellipodium; cytoplasmic microtubule; cytoplasm; plasma membrane; nucleus; lateral plasma membrane

Molecular Function: microtubule plus-end binding; protein binding; cadherin binding; microtubule binding; gamma-catenin binding; beta-catenin binding; protein kinase regulator activity; protein kinase binding

Biological Process: positive regulation of cell adhesion; somatic stem cell maintenance; positive regulation of apoptosis; apoptosis; positive regulation of microtubule polymerization; T cell differentiation in the thymus; Wnt receptor signaling pathway through beta-catenin; chromosome organization and biogenesis; cytoplasmic microtubule organization and biogenesis; anterior/posterior pattern formation; muscle maintenance; negative regulation of cell proliferation; regulation of osteoclast differentiation; cytokinesis after mitosis; hair follicle development; mitotic cell cycle spindle assembly checkpoint; positive regulation of epithelial cell differentiation; protein complex assembly; cell cycle arrest; cell adhesion; kidney development; proximal/distal pattern formation; cell structure disassembly during apoptosis; regulation of microtubule-based process; negative regulation of odontogenesis; regulation of attachment of spindle microtubules to kinetochore; cell migration; regulation of osteoblast differentiation; thymus development; negative regulation of microtubule depolymerization; negative regulation of MAPKKK cascade; axis specification; negative regulation of cyclin-dependent protein kinase activity; positive regulation of pseudopodium formation; dorsal/ventral pattern formation; axonogenesis; retina development in camera-type eye; positive regulation of cell division; positive regulation of protein catabolic process; regulation of nitrogen compound metabolic process; response to DNA damage stimulus; mitotic metaphase/anaphase transition; positive regulation of cell migration

Disease: Familial Adenomatous Polyposis 1; Gastric Cancer; Desmoid Disease, Hereditary; Colorectal Cancer; Hepatocellular Carcinoma