AP3S1 recombinant protein

AP3S1, 1-193aa, Human, His tag, E Coli

Cat. Num. AAA205223

• Gene Names: AP3S1; CLAPS3; Sigma3A
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: AP3S1; 1-193aa; Human; His tag; E Coli; AP-3 complex subunit sigma-1; CLAPS3; Sigma3A; AP3S1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL EGGLLIGGSD NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC FENVCELDLI FHVDKVHNIL AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS EAGLAGAPAR AVSAVKNMNL PEIPRNINIG DISIKVPNLP SFK
• Sequence Length: 193

• Applicable Applications for AP3S1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for AP3S1 recombinant protein

AP-3 complex subunit sigma-1, also known as AP3S1, is a Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Recombinant human AP3S1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for AP3S1 recombinant protein

Transportation

References

Burkard T.R., et al. (2011) BMC Syst. Biol. 5:17-17; Dell'Angelica E.C., et al. (1997) EMBO J. 16:917-928;

NCBI and Uniprot Product Information

• NCBI GI #: 4502861
• NCBI GeneID: 1176
• NCBI Accession #: NP_001275
• NCBI GenBank Nucleotide #: NM_001284.3
• UniProt Accession #: Q92572; O00647; O00676; O00721; O00727; Q53XL4; Q6ICQ2
• Molecular Weight: 23.8 kDa (213aa) confirmed by MALDI-TOF
• NCBI Official Full Name: AP-3 complex subunit sigma-1 isoform 1
• NCBI Official Synonym Full Names: adaptor related protein complex 3 sigma 1 subunit
• NCBI Official Symbol: AP3S1
• NCBI Official Synonym Symbols: CLAPS3; Sigma3A
• NCBI Protein Information: AP-3 complex subunit sigma-1
• UniProt Protein Name: AP-3 complex subunit sigma-1
• Protein Family: AP-3 complex
• UniProt Gene Name: AP3S1
• UniProt Synonym Gene Names: CLAPS3; Sigma3A-adaptin

NCBI Description

This gene encodes a subunit of the AP3 adaptor complex. This complex functions in the formation of subcellular vesicles budded from the Golgi body. Several related pseudogenes of this gene have been found. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]

Uniprot Description

Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.