Rabbit anti-Human, Mouse AP3S1 Polyclonal Antibody | anti-AP3S1 antibody

AP3S1, NT (AP3S1, CLAPS3, AP-3 complex subunit sigma-1, AP-3 complex subunit sigma-3A, Adapter-related protein complex 3 sigma-1 subunit, Clathrin-associated/assembly/adapter protein, small 3, Sigma-3A-adaptin, Sigma-adaptin 3a) (FITC)

Cat. Num. AAA6274129

• Gene Names: AP3S1; CLAPS3; Sigma3A
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: AP3S1; Polyclonal Antibody; NT (AP3S1; CLAPS3; AP-3 complex subunit sigma-1; AP-3 complex subunit sigma-3A; Adapter-related protein complex 3 sigma-1 subunit; Clathrin-associated/assembly/adapter protein; small 3; Sigma-3A-adaptin; Sigma-adaptin 3a) (FITC); Sigma-adaptin 3a; anti-AP3S1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-AP3S1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-100; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: AP3S1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-31 amino acids from the N-terminal region of human AP3S1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(AP3S1 Antibody (N-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).)

Western Blot (WB)

(AP3S1 Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the AP3S1 antibody detected the AP3S1 protein (arrow).)

Immunohistochemistry (IHC)

(AP3S1 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the AP3S1 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-AP3S1 antibody

Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes.

Product Categories/Family for anti-AP3S1 antibody

Antibodies; Clathrin

References

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Zhou, J.B., et al. Med. Sci. Monit. 16 (6), BR179-BR183 (2010): Lefrancois, S., et al. Dev. Cell 7(4):619-625(2004). Salazar, G., et al. Mol. Biol. Cell 15(2):575-587(2004). Nie, Z., et al. Dev. Cell 5(3):513-521(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 4502861
• NCBI GeneID: 1176
• NCBI Accession #: NP_001275.1
• NCBI GenBank Nucleotide #: NM_001284.2
• UniProt Accession #: Q92572; O00647; O00676; O00721; O00727; Q53XL4; Q6ICQ2
• Molecular Weight: 21,732 Da
• NCBI Official Full Name: AP-3 complex subunit sigma-1
• NCBI Official Synonym Full Names: adaptor-related protein complex 3, sigma 1 subunit
• NCBI Official Symbol: AP3S1
• NCBI Official Synonym Symbols: CLAPS3; Sigma3A
• NCBI Protein Information: AP-3 complex subunit sigma-1; AP-3 complex sigma-3A subunit; AP-3 complex subunit sigma-3A; adapter-related protein complex 3 sigma-1 subunit; adapter-related protein complex 3 subunit sigma-1; adaptor-related protein complex 3 subunit sigma-1; clathrin-a
• UniProt Protein Name: AP-3 complex subunit sigma-1
• Protein Family: AP-3 complex
• UniProt Gene Name: AP3S1
• UniProt Synonym Gene Names: CLAPS3; Sigma3A-adaptin
• UniProt Entry Name: AP3S1_HUMAN

Uniprot Description

AP3S1: Part of the AP-3 complex, an adapter-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. Belongs to the adaptor complexes small subunit family.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 5q22

Cellular Component: Golgi apparatus; transport vesicle; cytoplasmic vesicle membrane; AP-type membrane coat adaptor complex

Molecular Function: protein binding; transporter activity; protein transporter activity

Biological Process: intracellular protein transport; anterograde synaptic vesicle transport; insulin receptor signaling pathway; anterograde axon cargo transport