Annexin A4 (ANXA4) Recombinant Protein | ANXA4 recombinant protein

Recombinant Annexin A4 (ANXA4)

Cat. Num. AAA2011300

• Gene Names: ANXA4; ANX4; PIG28; ZAP36
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Annexin A4 (ANXA4); Recombinant Annexin A4 (ANXA4); ANXA4 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-KAASGFNAME DAQTLRKAMK GLGTDEDAII SVLAYRNTAQ RQEIRTAYKS TIGRDLIDDL KSELSGNFEQ VIVGMMTPTV LYDVQELRRA MKGAGTDEGC LIEILASRTP EEIRRISQTY QQQYGRSLED DIRSDTSFMF QRVLVSLSAG GRDE
• Sequence Length: 2158

• Applicable Applications for ANXA4 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Lys11~Glu164 (Accession # NM_001153.3) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 168480078
• NCBI GeneID: 307
• NCBI Accession #: NP_001144.1
• NCBI GenBank Nucleotide #: NM_001153.3
• UniProt Accession #: P09525; Q96F33; Q9BWK1
• Molecular Weight: 18.7kDa
• NCBI Official Full Name: Homo sapiens annexin A4 (ANXA4), mRNA
• NCBI Official Synonym Full Names: annexin A4
• NCBI Official Symbol: ANXA4
• NCBI Official Synonym Symbols: ANX4; PIG28; ZAP36
• NCBI Protein Information: annexin A4; P32.5; PP4-X; PAP-II; annexin-4; protein II; endonexin I; lipocortin IV; chromobindin-4; 35-beta calcimedin; proliferation-inducing gene 28; proliferation-inducing protein 28; placental anticoagulant protein II; carbohydrate-binding protein p33/p41; annexin IV (placental anticoagulant protein II)
• UniProt Protein Name: Annexin A4
• Protein Family: Annexin
• UniProt Gene Name: ANXA4
• UniProt Synonym Gene Names: ANX4; PAP-II
• UniProt Entry Name: ANXA4_HUMAN

NCBI Description

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA4: a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding; Lipid-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nuclear membrane; cell surface; perinuclear region of cytoplasm; cytoplasm; plasma membrane; vesicle membrane; nucleus

Molecular Function: NF-kappaB binding; phospholipase inhibitor activity; identical protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; epithelial cell differentiation; inhibition of NF-kappaB transcription factor; negative regulation of catalytic activity; signal transduction; negative regulation of apoptosis