Mouse Annexin IV (ANX- IV) ELISA Kit | ANXA4 elisa kit

Mouse Annexin IV (ANX- IV) ELISA Kit

Cat. Num. AAA7209457

• Gene Names: ANXA4; ANX4; PIG28; ZAP36; HEL-S-274
• Reactivity: Mouse
• Synonyms: Annexin IV (ANX- IV); Mouse Annexin IV (ANX- IV) ELISA Kit; ANXA4 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 10-250ng/mL
• Sensitivity: 1.0ng/mL
• Intended Uses: This ANX- IV ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ANX- IV. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA4 elisa kit

Principle of the assay: ANX- IV ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ANX- IV antibody and an ANX- IV-HRP conjugate. The assay sample and buffer are incubated together with ANX- IV-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ANX- IV concentration since ANX- IV from samples and ANX- IV-HRP conjugate compete for the anti-ANX- IV antibody binding site. Since the number of sites is limited, as more sites are occupied by ANX- IV from the sample, fewer sites are left to bind ANX- IV-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ANX- IV concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ANXA4 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 1703319
• NCBI GeneID: 307
• Molecular Weight: 35,883 Da
• NCBI Official Full Name: Annexin A4
• NCBI Official Synonym Full Names: annexin A4
• NCBI Official Symbol: ANXA4
• NCBI Official Synonym Symbols: ANX4; PIG28; ZAP36; HEL-S-274
• NCBI Protein Information: annexin A4; P32.5; PP4-X; PAP-II; annexin-4; protein II; endonexin I; lipocortin IV; chromobindin-4; 35-beta calcimedin; proliferation-inducing gene 28; proliferation-inducing protein 28; placental anticoagulant protein II; epididymis secretory protein Li 274; carbohydrate-binding protein p33/p41; annexin IV (placental anticoagulant protein II)
• UniProt Protein Name: Annexin A4
• Protein Family: Annexin
• UniProt Gene Name: ANXA4
• UniProt Synonym Gene Names: ANX4; PAP-II
• UniProt Entry Name: ANXA4_HUMAN

NCBI Description

Annexin IV (ANX4) belongs to the annexin family of calcium-dependent phospholipid binding proteins. Although their functions are still not clearly defined, several members of the annexin family have been implicated in membrane-related events along exocytotic and endocytotic pathways. ANX4 has 45 to 59% identity with other members of its family and shares a similar size and exon-intron organization. Isolated from human placenta, ANX4 encodes a protein that has possible interactions with ATP, and has in vitro anticoagulant activity and also inhibits phospholipase A2 activity. ANX4 is almost exclusively expressed in epithelial cells. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA4: a calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Calcium-binding

Chromosomal Location of Human Ortholog: 2p13

Cellular Component: nuclear membrane; cell surface; perinuclear region of cytoplasm; cytoplasm; plasma membrane; vesicle membrane; nucleus

Molecular Function: phospholipase inhibitor activity; NF-kappaB binding; identical protein binding; calcium-dependent phospholipid binding; calcium ion binding; calcium-dependent protein binding

Biological Process: regulation of transcription from RNA polymerase II promoter; epithelial cell differentiation; inhibition of NF-kappaB transcription factor; negative regulation of catalytic activity; signal transduction; negative regulation of apoptosis