SDS-Page
(15% SDS-PAGE using A2295-15B2 (3ug).)
Annexin A2 Recombinant Protein | ANXA2 recombinant protein
Annexin A2, Recombinant, Human
Applications
Western Blot
Purity
Affinity Purified~95% by SDS-PAGE Concentration: ~0.5mg/ml
Synonyms
Annexin A2; Recombinant; Human; ANXA2 recombinant protein
Host
E Coli
Purity/Purification
Affinity Purified
~95% by SDS-PAGE Concentration: ~0.5mg/ml
~95% by SDS-PAGE Concentration: ~0.5mg/ml
Form/Format
Supplied as a liquid in 20mM Tris-HCl buffer, pH8.0, 10% glycerol, 0.1M sodium chloride
Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMST VHEILCKLSL EGDHSTPPSA YGSVKAYTNF DAERDALNIE TAIKTKGVDE VTIVNILTNR SNAQRQDIAF AYQRRTKKEL ASALKSALSG HLETVILGLL KTPAQYDASE LKASMKGLGT DEDSLIEIIC SRTNQELQEI NRVYKEMYKT DLEKDIISDT SGDFRKLMVA LAKGRRAEDG SVIDYELIDQ DARDLYDAGV KRKGTDVPKW ISIMTERSVP HLQKVFDRYK SYSPYDMLES IRKEVKGDLE NAFLNLVQCI QNKPLYFADR LYDSMKGKGT RDKVLIRIMV SRSEVDMLKI RSEFKRKYGK SLYYYIQQDT KGDYQKALLY LCGGDD
Applicable Applications for ANXA2 recombinant protein
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(15% SDS-PAGE using A2295-15B2 (3ug).)
SDS-Page
(15% SDS-PAGE using A2295-15B2 (3ug).)
Related Product Information for ANXA2 recombinant protein
Annexin A2 belongs to a family of calcium-dependent membrane and phospholipid binding proteins. It plays a role in the regulation of cellular growth and in signal transduction pathways. This protein functions as an autocrine factor which heightens osteoclast formation and bone resorption.
Recombinant Annexin A2 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Recombinant Annexin A2 protein was expressed in E.coli and purified by using conventional chromatography techniques.
Product Categories/Family for ANXA2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
42.8kD
NCBI Official Full Name
annexin A2
NCBI Official Symbol
ANXA2
NCBI Protein Information
annexin A2; p36; PAP-IV; annexin-2; protein I; annexin II; lipocortin II; chromobindin-8; calpactin I heavy chain; calpactin-1 heavy chain; placental anticoagulant protein IV
UniProt Protein Name
Annexin A2
Protein Family
UniProt Gene Name
ANXA2
UniProt Synonym Gene Names
ANX2; PAP-IV
UniProt Entry Name
ANXA2_PIG
Uniprot Description
Function: Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity.
Subunit structure: Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with ATP1B1 and DYSF
By similarity. Interacts with COCH
By similarity.
Subcellular location: Secreted › extracellular space › extracellular matrix › basement membrane. Melanosome
By similarity. Note: In the lamina beneath the plasma membrane.
Domain: A pair of annexin repeats may form one binding site for calcium and phospholipid.
Post-translational modification: ISGylated
By similarity.
Miscellaneous: It may cross-link plasma membrane phospholipids with actin and the cytoskeleton and be involved with exocytosis.
Sequence similarities: Belongs to the annexin family.Contains 4 annexin repeats.
Research Articles on ANXA2
Similar Products
Product Notes
The ANXA2 anxa2 (Catalog #AAA638184) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Annexin A2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Researchers should empirically determine the suitability of the ANXA2 anxa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMST VHEILCKLSL EGDHSTPPSA YGSVKAYTNF DAERDALNIE TAIKTKGVDE VTIVNILTNR SNAQRQDIAF AYQRRTKKEL ASALKSALSG HLETVILGLL KTPAQYDASE LKASMKGLGT DEDSLIEIIC SRTNQELQEI NRVYKEMYKT DLEKDIISDT SGDFRKLMVA LAKGRRAEDG SVIDYELIDQ DARDLYDAGV KRKGTDVPKW ISIMTERSVP HLQKVFDRYK SYSPYDMLES IRKEVKGDLE NAFLNLVQCI QNKPLYFADR LYDSMKGKGT RDKVLIRIMV SRSEVDMLKI RSEFKRKYGK SLYYYIQQDT KGDYQKALLY LCGGDD. It is sometimes possible for the material contained within the vial of "Annexin A2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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