Mouse annexin A1 ELISA Kit | ANXA1 elisa kit

Mouse Annexin A1, ANXA1 ELISA Kit

Cat. Num. AAA944251

• Gene Names: Anxa1; Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik
• Reactivity: Mouse
• Synonyms: annexin A1; Mouse Annexin A1; ANXA1 ELISA Kit; Mouse Annexin A1 (ANXA1) ELISA kit; ANX1; LPC1; OTTHUMP00000021475; annexin I; annexin I (lipocortin I) ; lipocortin I; ANXA1 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ANX-VII. No significant cross-reactivity or interference between human ANX-VII and analogues was observed.
• Sequence Length: 346

• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.25 ng/ml-80 ng/ml
• Sensitivity: 0.31 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ANXA1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANX-VII has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANX-VII present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANX-VII is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANX-VII bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 124517663
• NCBI GeneID: 16952
• NCBI Accession #: NP_034860.2
• NCBI GenBank Nucleotide #: NM_010730.2
• UniProt Accession #: P10107
• Molecular Weight: 38,734 Da
• NCBI Official Full Name: annexin A1
• NCBI Official Synonym Full Names: annexin A1
• NCBI Official Symbol: Anxa1
• NCBI Official Synonym Symbols: Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik
• NCBI Protein Information: annexin A1; p35; annexin I; annexin-1; calpactin-2; calpactin II; lipocortin 1; lipocortin I; chromobindin-9; phospholipase A2 inhibitory protein
• UniProt Protein Name: Annexin A1
• Protein Family: Annexin
• UniProt Gene Name: Anxa1
• UniProt Synonym Gene Names: Anx1; Lpc-1; Lpc1
• UniProt Entry Name: ANXA1_MOUSE

Uniprot Description

ANXA1: a calcium/phospholipid-binding protein with which promotes membrane fusion and is involved in endocytosis. Has anti-inflammatory properties and inhibits phospholipase A2 activity. Accumulates on internalized vesicles after EGF-stimulated endocytosis, suggesting that it may be required for a late stage in inward vesiculation. Phosphorylated by PKC, EGFR and Chak1. Phosphorylation results in loss of the inhibitory activity. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Lipid-binding; Calcium-binding

Cellular Component: extracellular space; focal adhesion; protein complex; mast cell granule; cell surface; cornified envelope; cilium; cell projection; membrane; cytoplasm; mitochondrial membrane; plasma membrane; nucleus; vesicle; endosome; sarcolemma

Molecular Function: protein binding, bridging; phospholipase inhibitor activity; protein binding; protein homodimerization activity; single-stranded RNA binding; calcium-dependent phospholipid binding; phospholipase A2 inhibitor activity; double-stranded DNA-dependent ATPase activity; phospholipid binding; calcium ion binding; structural molecule activity; calcium-dependent protein binding; helicase activity; single-stranded DNA binding

Biological Process: negative regulation of protein secretion; positive regulation of neutrophil apoptosis; positive regulation of apoptosis; alpha-beta T cell differentiation; positive regulation of vesicle fusion; neutrophil homeostasis; signal transduction; arachidonic acid secretion; DNA duplex unwinding; regulation of cell proliferation; keratinocyte differentiation; cell surface receptor linked signal transduction; insulin secretion; DNA strand renaturation; peptide cross-linking; positive regulation of prostaglandin biosynthetic process