ANTXR1 recombinant protein

Recombinant Human ANTXR1 Protein

Cat. Num. AAA8248538

• Gene Names: ANTXR1; ATR; GAPO; TEM8
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: ANTXR1; Recombinant Human ANTXR1 Protein; ATR; TEM8; Anthrax toxin receptor 1; Tumor endothelial marker 8; ANTXR1 recombinant protein

• Host: Human cells
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of 20mM PB, 150mM NaCl, pH 7.4.
• Sequence: EDGGPACYGG FDLYFILDKS GSVLHHWNEI YYFVEQLAHK FISPQLRMSF IVFSTRGTTL MKLTEDREQI RQGLEELQKV LPGGDTYMHE GFERASEQIY YENRQGYRTA SVIIALTDGE LHEDLFFYSE REANRSRDLG AIVYCVGVKD FNETQLARIA DSKDHVFPVN DGFQALQGII HSILKKSCIE ILAAEPSTIC AGESFQVVVR GNGFRHARNV DRVLCSFKIN DSVTLNEKPF SVEDTYLLCP APILKEVGMK AALQVSMNDG LSFISSSVII TTTHCSLHKV DHHHHHH
• Sequence Length: 333

• Applicable Applications for ANTXR1 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for ANTXR1 recombinant protein

Recombinant Human ANTXR1 Protein is produced by our mammalian expression system and the target gene encoding Glu33-Lys321 is expressed with a 6His tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 16933553
• NCBI GeneID: 84168
• NCBI Accession #: NP_060623.2
• NCBI GenBank Nucleotide #: NM_018153.3
• UniProt Accession #: Q9H6X2; Q4ZFV6; Q53QD8; Q96P02; Q9NVP3; A8K7U8; J7K7G4; J7KF88
• Molecular Weight: 39,742 Da
• NCBI Official Full Name: anthrax toxin receptor 1 isoform 3
• NCBI Official Synonym Full Names: anthrax toxin receptor 1
• NCBI Official Symbol: ANTXR1
• NCBI Official Synonym Symbols: ATR; GAPO; TEM8
• NCBI Protein Information: anthrax toxin receptor 1
• UniProt Protein Name: Anthrax toxin receptor 1
• Protein Family: Anthrax toxin receptor
• UniProt Gene Name: ANTXR1
• UniProt Synonym Gene Names: ATR; TEM8
• UniProt Entry Name: ANTR1_HUMAN

NCBI Description

This gene encodes a type I transmembrane protein and is a tumor-specific endothelial marker that has been implicated in colorectal cancer. The encoded protein has been shown to also be a docking protein or receptor for Bacillus anthracis toxin, the causative agent of the disease, anthrax. The binding of the protective antigen (PA) component, of the tripartite anthrax toxin, to this receptor protein mediates delivery of toxin components to the cytosol of cells. Once inside the cell, the other two components of anthrax toxin, edema factor (EF) and lethal factor (LF) disrupt normal cellular processes. Three alternatively spliced variants that encode different protein isoforms have been described. [provided by RefSeq, Oct 2008]

Uniprot Description

ANTXR1: Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells. Defects in ANTXR1 are associated with susceptibility to hemangioma capillary infantile (HCI). HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. Belongs to the ATR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Actin-binding; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cell surface; endosome membrane; external side of plasma membrane; filopodium membrane; plasma membrane

Molecular Function: actin filament binding; collagen binding; protein binding; transmembrane receptor activity

Biological Process: actin cytoskeleton reorganization

Disease: Gapo Syndrome; Hemangioma, Capillary Infantile