GCNT3 Antibody | anti-GCNT3 antibody

GCNT3, CT (GCNT3, Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3, C2GnT-mucin type, Core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase) (AP)

Cat. Num. AAA6301698

• Gene Names: GCNT3; GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified by Protein A and peptide affinity chromatography.
• Synonyms: GCNT3; CT (GCNT3; Beta-1; 3-galactosyl-O-glycosyl-glycoprotein beta-1; 6-N-acetylglucosaminyltransferase 3; C2GnT-mucin type; Core 2/core 4 beta-1; 6-N-acetylglucosaminyltransferase) (AP); 6-N-acetylglucosaminyltransferase; anti-GCNT3 antibody

• Isotype: IgG
• Specificity: Recognizes human GCNT3. Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A and peptide affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeledwith Alkaline Phophatase (AP).
• Concentration: 0.45mg/ml (varies by lot)

• Applicable Applications for anti-GCNT3 antibody: Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
• Application Notes: Immunohistochemistry: Formalin-fixed, paraffin-embeddedsections; Other applications have not been tested. Optimal dilutions to be determined by the researcher.
• Grade: Affinity Purified
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 390-418 in the C-terminal region ofhuman GCNT3. Species Sequence Homology: rat
• Preparation and Storage: Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can bemade in assay buffer. For maximum recovery of product,centrifuge the original vial prior to removing the cap.; ; Note: Applications are based on unconjugated antibody.

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane)using MBS6301698 This demonstrates that 035969 detected the GCNT3protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed paraffin embeddedhuman colon carcinoma using MBS6301698 followed by peroxida seconjugatied secondary antibody and DAB staining. This datademonstrates the use of MBS6301698 for immunohistochemistry.)

Related Product Information for anti-GCNT3 antibody

This gene encodes a member of theN-acetylglucosaminyltransferase family. The encoded protein isa beta-6-N-acetylglucosamine-transferase that catalyzes theformation of core 2 and core 4 O-glycans on mucin-typeglycoproteins.

Product Categories/Family for anti-GCNT3 antibody

Antibodies; Enzymes; Glycosyltransferase

References

Tan, S., et al. Am. J. Respir. Cell Mol. Biol. 36(6):737-745(2007). Lamesch, P., et al. Genomics 89(3):307-315(2007). Huang, M.C., et al. Oncogene 25(23):3267-3276(2006). Beum, P.V., et al. Am. J. Respir. Cell Mol. Biol. 29(1):48-56(2003). Schwientek, T., et al. J. Biol. Chem. 274(8):4504-4512(1999).

NCBI and Uniprot Product Information

• NCBI GI #: 4758422
• NCBI GeneID: 9245
• NCBI Accession #: NP_004742.1
• NCBI GenBank Nucleotide #: NM_004751.2
• UniProt Accession #: O95395
• NCBI Official Full Name: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
• NCBI Official Synonym Full Names: glucosaminyl (N-acetyl) transferase 3, mucin type
• NCBI Official Symbol: GCNT3
• NCBI Official Synonym Symbols: GNTM; C24GNT; C2GNT2; C2GNTM; C2/4GnT
• NCBI Protein Information: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3; hC2GnT-M; C2GnT-mucin type; beta1,6GlcNAc-transferase; beta1,6-N-acetylglucosaminyltransferase-M; core 2/core 4 beta-1,6-N-acetylglucosaminyltransferase; mucus-type co
• UniProt Protein Name: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 3
• Protein Family: Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase
• UniProt Gene Name: GCNT3
• UniProt Synonym Gene Names: C2GnT-M; hC2GnT-M; C2/4GnT
• UniProt Entry Name: GCNT3_HUMAN

NCBI Description

This gene encodes a member of the N-acetylglucosaminyltransferase family. The encoded protein is a beta-6-N-acetylglucosamine-transferase that catalyzes the formation of core 2 and core 4 O-glycans on mucin-type glycoproteins.[provided by RefSeq, Apr 2009]

Uniprot Description

GCNT3: Glycosyltransferase that can synthesize all known mucin beta 6 N-acetylglucosaminides. Mediates core 2 and core 4 O-glycan branching, 2 important steps in mucin-type biosynthesis. Has also I-branching enzyme activity by converting linear into branched poly-N-acetyllactosaminoglycans, leading to introduce the blood group I antigen during embryonic development. Belongs to the glycosyltransferase 14 family.

Protein type: EC 2.4.1.150; Transferase; EC 2.4.1.102; Membrane protein, integral; Glycan Metabolism - O-glycan biosynthesis

Chromosomal Location of Human Ortholog: 15q21.3

Cellular Component: Golgi membrane; membrane; integral to membrane

Molecular Function: beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity; N-acetyllactosaminide beta-1,6-N-acetylglucosaminyltransferase activity; acetylgalactosaminyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase activity

Biological Process: protein amino acid O-linked glycosylation; cellular protein metabolic process; tissue morphogenesis; O-glycan processing; immunoglobulin production in mucosal tissue; carbohydrate metabolic process; post-translational protein modification; intestinal absorption