Western Blot (WB)
(Western blot analysis of whole cell lysates probed with C9orf72 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP with 73 second exposure. Arrow points to C9orf72 (molecular weight 54 kDa))
C9orf72 Antibody | anti-C9orf72 antibody
C9orf72 Antibody with Control Lysate
Mouse anti Human C9orf72 antibody recognizes the protein C9orf72
Expansion of a hexanucleotide repeat in non-coding sequence between alternate 5' exons in transcripts from C9orf72 is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Multiple transcript variants encoding different isoforms have been found for C9orf72. (provided by RefSeq, Dec 2011).
Mouse anti Human C9orf72 antibody detects a band of 54 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Western Blotting: Anti C9orf72 detects a band of approximately 54 kDa in HEK293 cell lysates
N.B. Antibody reactivity and working conditions may vary between species.
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with C9orf72 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP with 73 second exposure. Arrow points to C9orf72 (molecular weight 54 kDa))
Western Blot (WB)
(Western blot analysis of whole cell lysates probed with C9orf72 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/5,000) and visualized on the ChemiDoc MP with 73 second exposure. Arrow points to C9orf72 (molecular weight 54 kDa))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene plays an important role in the regulation of endosomal trafficking, and has been shown to interact with Rab proteins that are involved in autophagy and endocytic transport. Expansion of a GGGGCC repeat from 2-22 copies to 700-1600 copies in the intronic sequence between alternate 5' exons in transcripts from this gene is associated with 9p-linked ALS (amyotrophic lateral sclerosis) and FTD (frontotemporal dementia) (PMID: 21944778, 21944779). Studies suggest that hexanucleotide expansions could result in the selective stabilization of repeat-containing pre-mRNA, and the accumulation of insoluble dipeptide repeat protein aggregates that could be pathogenic in FTD-ALS patients (PMID: 23393093). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2016]
Uniprot Description
Component of the C9orf72-SMCR8 complex, a complex that has guanine nucleotide exchange factor (GEF) activity and regulates autophagy (PubMed:27193190, PubMed:27103069, PubMed:27617292, PubMed:28195531). In the complex, C9orf72 and SMCR8 probably constitute the catalytic subunits that promote the exchange of GDP to GTP, converting inactive GDP-bound RAB8A and RAB39B into their active GTP-bound form, thereby promoting autophagosome maturation (PubMed:27103069). The C9orf72-SMCR8 complex also acts as a regulator of autophagy initiation by interacting with the ATG1/ULK1 kinase complex and modulating its protein kinase activity (PubMed:27617292). Positively regulates initiation of autophagy by regulating the RAB1A-dependent trafficking of the ATG1/ULK1 kinase complex to the phagophore which leads to autophagosome formation (PubMed:27334615). Acts as a regulator of mTORC1 signaling by promoting phosphorylation of mTORC1 substrates (PubMed:27559131). Plays a role in endosomal trafficking (PubMed:24549040). May be involved in regulating the maturation of phagosomes to lysosomes (). Regulates actin dynamics in motor neurons by inhibiting the GTP-binding activity of ARF6, leading to ARF6 inactivation (PubMed:27723745). This reduces the activity of the LIMK1 and LIMK2 kinases which are responsible for phosphorylation and inactivation of cofilin, leading to cofilin activation (PubMed:27723745). Positively regulates axon extension and axon growth cone size in spinal motor neurons (PubMed:27723745). Plays a role within the hematopoietic system in restricting inflammation and the development of autoimmunity ().
Research Articles on C9orf72
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Product Notes
The C9orf72 c9orf72 (Catalog #AAA225436) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's C9orf72 can be used in a range of immunoassay formats including, but not limited to, Western Blotting (WB). WB: 1/1000 Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type. Western Blotting: Anti C9orf72 detects a band of approximately 54 kDa in HEK293 cell lysates. Researchers should empirically determine the suitability of the C9orf72 c9orf72 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "C9orf72, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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