c-Kit antibody kit

c-Kit Antibody Sampler Kit

Cat. Num. AAA2550059

• Gene Names: KIT; PBT; SCFR; C-Kit; CD117
• Applications: Western Blot
• Synonyms: c-Kit; c-Kit Antibody Sampler Kit; c-Kit antibody kit

• Sequence Length: 22

• Applicable Applications for c-Kit antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of 453 cells using c-Kit Polyclonal Antibody at dilution of 1:500.)

Western Blot (WB)

(Western Blot analysis of HepG2 cells with Phospho-c-Kit (Tyr721) Polyclonal Antibody)

Related Product Information for c-Kit antibody kit

Background: c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/flk-2 receptors. It plays a critical role in activation and growth in a number of cell types including hematopoietic stem cells, mast cells, melanocytes, and germ cells. Upon binding with its stem cell factor (SCF) ligand, c-Kit undergoes dimerization/oligomerization and autophosphorylation. Activation of c- Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCgamma, the p85 subunit of PI3 kinase, SHP2, and CrkL. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, and mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors. Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 459359
• NCBI GeneID: 3815
• Molecular Weight: 46,658 Da
• NCBI Official Full Name: c-Kit, partial
• NCBI Official Synonym Full Names: KIT proto-oncogene receptor tyrosine kinase
• NCBI Official Symbol: KIT
• NCBI Official Synonym Symbols: PBT; SCFR; C-Kit; CD117
• NCBI Protein Information: mast/stem cell growth factor receptor Kit
• UniProt Protein Name: Mast/stem cell growth factor receptor Kit
• UniProt Gene Name: KIT
• UniProt Synonym Gene Names: SCFR; SCFR; PBT

NCBI Description

This gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Kit: a receptor tyrosine kinase and a member of the subfamily that includes PDGF, CSF-1 and FLT-3/flk-2 receptors. Receptor for stem cell factor. Plays a critical role in hematopoietic stem cell, mast cell, melanocyte and germ cell development. Ligand binding induces autophosphorylation, dimerization and activation, leading to the recruitment and phosphorylation of downstream SH2-containing signaling components including PLC-gamma, PI3 kinase p85, SHP2 and CrkL, linking c-Kit to various cell signaling pathways. Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders, while mutations that constitutively activate c-Kit can lead to hyperplasia and tumorigenesis. Activating mutations cause >90% of gastrointestinal stromal tumors (GIST); successfully treated with inhibitors Gleevec (imatinib, Glivec) and Sutent (Sutinib, SU11248). Activating mutations also induce mastocytosis. Autocrine/paracrine stimulation may drive some lung and other tumors. Loss of expression associated with melanoma progression. Familial loss of function mutations cause piebaldism, with defects in hair and skin pigmentation due to lack of melanocytes.

Protein type: EC 2.7.10.1; Kinase, protein; Membrane protein, integral; Oncoprotein; PDGFR family; Protein kinase, TK; Protein kinase, tyrosine (receptor); TK group

Chromosomal Location of Human Ortholog: 4q12

Cellular Component: acrosomal vesicle; cytoplasmic side of plasma membrane; external side of plasma membrane; extracellular space; integral component of membrane; intercellular junction; mast cell granule; plasma membrane

Molecular Function: ATP binding; cytokine binding; metal ion binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protease binding; protein binding; protein homodimerization activity; protein tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; signal transducer, downstream of receptor, with protein tyrosine kinase activity; stem cell factor receptor activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: actin cytoskeleton reorganization; activation of MAPK activity; cytokine-mediated signaling pathway; dendritic cell cytokine production; detection of mechanical stimulus involved in sensory perception of sound; embryonic hemopoiesis; epithelial cell proliferation; erythrocyte differentiation; erythropoietin-mediated signaling pathway; Fc receptor signaling pathway; germ cell migration; germ cell programmed cell death; glycosphingolipid metabolic process; gut development; hemopoiesis; immature B cell differentiation; inflammatory response; Kit signaling pathway; lamellipodium assembly; lymphoid progenitor cell differentiation; male gonad development; MAPK cascade; mast cell chemotaxis; mast cell cytokine production; mast cell degranulation; melanocyte adhesion; melanocyte differentiation; melanocyte migration; myeloid progenitor cell differentiation; negative regulation of programmed cell death; ovarian follicle development; peptidyl-tyrosine phosphorylation; phosphatidylinositol phosphorylation; pigmentation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of colon smooth muscle contraction; positive regulation of gene expression; positive regulation of JAK-STAT cascade; positive regulation of long-term neuronal synaptic plasticity; positive regulation of MAPK cascade; positive regulation of Notch signaling pathway; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of phospholipase C activity; positive regulation of protein kinase B signaling; positive regulation of pseudopodium assembly; positive regulation of pyloric antrum smooth muscle contraction; positive regulation of small intestine smooth muscle contraction; positive regulation of transcription factor activity; positive regulation of tyrosine phosphorylation of STAT protein; positive regulation of vascular smooth muscle cell differentiation; protein autophosphorylation; regulation of bile acid metabolic process; regulation of cell proliferation; regulation of cell shape; regulation of developmental pigmentation; regulation of transcription from RNA polymerase II promoter; response to cadmium ion; signal transduction; somatic stem cell division; somatic stem cell maintenance; spermatid development; spermatogenesis; stem cell differentiation; stem cell maintenance; T cell differentiation; tongue development; visual learning

Disease: Gastrointestinal Stromal Tumor; Mast Cell Disease; Piebald Trait; Testicular Germ Cell Tumor