APN recombinant protein

Recombinant Mouse APN Protein (His Tag)

Cat. Num. AAA2563879

• Gene Names: Anpep; Apn; AP-M; AP-N; Cd13; P150
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: APN; Recombinant Mouse APN Protein (His Tag); Aminopeptidase N; ANPEP; AP-M; AP-N; CD13 antigen; CD13; CD13APN; PEPN; PEPNhAPN; APN recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 um Filtered Solution of PBS, pH 7.4.
• Sequence Positions: Lys69-Ser966
• Sequence Length: 966

• Species: Human
• Endotoxin: < 1.0 EU per ug as determined by the LAL method.
• Fusion Tag: C-6His
• Preparation and Storage: Lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for APN recombinant protein

Protein Construction: Recombinant Mouse Aminopeptidase N is produced by our Mammalian expression system and the target gene encoding Lys69-Ser966 is expressed with a 6His tag at the C-terminus.

Background: ANPEP gene encodes aminopeptidase N (APN) also known as microsomal aminopeptiase, alanyl aminopeptidase, aminopeptidase M, CD13, or membrane protein p161, is a member of the peptidase M1 family. Widely expressed in many cells, tissues and species, APN cleaves the N-terminal amino acids from bioactive peptides, leading to their inactivation or degradation. Probably plays a role in regulating growth and differentiation of early B-lineage cells. It also may play a role in the catabolic pathway of the renin-angiotensin system. It degrades vasoconstricting angiotensin II into angiotensin III and therefore helps to regulate blood pressure.

NCBI and Uniprot Product Information

• NCBI GI #: 31077182
• NCBI GeneID: 16790
• UniProt Accession #: P97449
• Molecular Weight: Molecular Mass: 103.6 kDa; Actual Protein Molecular Mass: 110-130 kDa
• NCBI Official Full Name: Aminopeptidase N
• NCBI Official Synonym Full Names: alanyl (membrane) aminopeptidase
• NCBI Official Symbol: Anpep
• NCBI Official Synonym Symbols: Apn; AP-M; AP-N; Cd13; P150
• NCBI Protein Information: aminopeptidase N
• UniProt Protein Name: Aminopeptidase N
• UniProt Gene Name: Anpep
• UniProt Synonym Gene Names: Lap-1; Lap1; AP-N; mAPN; AP-M
• UniProt Entry Name: AMPN_MOUSE

Uniprot Description

CD13: Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. Belongs to the peptidase M1 family.

Protein type: EC 3.4.11.2; Protease; Receptor, misc.; Other Amino Acids Metabolism - glutathione; Membrane protein, integral

Cellular Component: extracellular space; membrane; lysosomal membrane; integral to membrane; ER-Golgi intermediate compartment; vesicle membrane; external side of plasma membrane

Molecular Function: peptidase activity; metallopeptidase activity; zinc ion binding; hydrolase activity; metal ion binding; aminopeptidase activity; peptide binding

Biological Process: multicellular organismal development; aromatic compound metabolic process; angiogenesis; cell differentiation; proteolysis