ANGPTL2 recombinant protein

ANGPTL2 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120630

• Gene Names: ANGPTL2; ARP2; HARP
• Purity: >95% as determined by SDS-PAGE
• Synonyms: ANGPTL2; ANGPTL2 Protein; Human; Recombinant (His Tag); Human ANGPTL2/Angiopoietin-like 2 Protein (His Tag); ARP2 Protein; HARP Protein; angiopoietin-like 2; ANGPTL2 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Asp245-His493

• Species: Human
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the human ANGPTL2 (NP_036230.1)(Asp245-His493) was expressed with a polyhistidine tag at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for ANGPTL2 recombinant protein

Background: The angiopoietin-like protein (ANGPTL) family is homologous to angiopoietins but does not bind to the Tie2 receptor. The function of ANGPTLs has been elucidated largely in the context of angiogenesis and lipid metabolism. Morinaga et al. demonstrated that genetic depletion of Angptl2 confers amelioration of the mouse kidney fibrosis induced by a unilateral ureteral obstruction, implicating that ANGPTL2, predominantly in the renal tubular compartments, activates the transforming growth factor-beta signaling and vice versa through miR-221. Angiopoietin-like protein 2 (ANGPTL2) maintains tissue homeostasis by inducing inflammation and angiogenesis. It is produced in infiltrating immune cells or resident cells, such as adipocytes, vascular endothelial cells, and tumor cells. The classic sequential cascade of P. gingivalis LPS --> inflammatory cytokine induction is well established. However, in the current study, we reveal a novel cascade comprising sequential P. gingivalis LPS --> ANGPTL2 --> integrin alpha5beta1 --> inflammatory cytokine induction, which might be responsible for inducing potent periodontal disorganization activity in gingival epithelial cells. Via this pathway, ANGPTL2 functions in the pathogenesis of periodontitis and contributes to prolonging chronic inflammation in patients with systemic disease. That MAC-3-positive immune cells, including infiltrating bone marrow-derived macrophages and activated microglia, express abundant angiopoietin-like protein (ANGPTL) 2 in ischemic mouse brain in a transient middle cerebral artery occlusion (MCAO) model. Both neurological deficits and infarct volume decreased in transient MCAO model mice established in Angptl2 knockout (KO) relative to wild-type mice. Acute brain inflammation after ischemia-reperfusion, as estimated by expression levels of pro-inflammatory cytokines such as interleukin (IL)-1beta and tumor necrosis factor alpha (TNF)-alpha, was significantly suppressed in Angptl2 KO compared to control mice.

NCBI and Uniprot Product Information

• NCBI GI #: 6912236
• NCBI GeneID: 23452
• NCBI Accession #: NP_036230.1
• NCBI GenBank Nucleotide #: NM_012098.2
• UniProt Accession #: Q9UKU9; Q5JT58; Q8NCH7
• Molecular Weight: 22,717 Da
• NCBI Official Full Name: angiopoietin-related protein 2
• NCBI Official Synonym Full Names: angiopoietin-like 2
• NCBI Official Symbol: ANGPTL2
• NCBI Official Synonym Symbols: ARP2; HARP
• NCBI Protein Information: angiopoietin-related protein 2; angiopoietin-like protein 2
• UniProt Protein Name: Angiopoietin-related protein 2
• Protein Family: Angiopoietin-related protein
• UniProt Gene Name: ANGPTL2
• UniProt Synonym Gene Names: ARP2
• UniProt Entry Name: ANGL2_HUMAN

NCBI Description

Angiopoietins are members of the vascular endothelial growth factor family and the only known growth factors largely specific for vascular endothelium. Angiopoietin-1, angiopoietin-2, and angiopoietin-4 participate in the formation of blood vessels. ANGPTL2 protein is a secreted glycoprotein with homology to the angiopoietins and may exert a function on endothelial cells through autocrine or paracrine action. [provided by RefSeq, Jul 2008]

Uniprot Description

ANGPTL2: Induces sprouting in endothelial cells through an autocrine and paracrine action.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: extracellular space

Molecular Function: receptor binding

Biological Process: multicellular organismal development