Angiopoietin 1 (ANGPT1) Recombinant Protein | ANGPT1 recombinant protein

Recombinant Angiopoietin 1 (ANGPT1)

Cat. Num. AAA2011230

• Gene Names: Angpt1; Ang1; Ang-1; 1110046O21Rik
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Angiopoietin 1 (ANGPT1); Recombinant Angiopoietin 1 (ANGPT1); ANGPT1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGS-RIQHGQ CAYTFILPEH DGNCRESATE QYNTNALQRD APHVEPDFSS QKLQHLEHVM ENYTQWLQKL ENYIVENMKS EMAQIQQNAV QNHTATMLEI GTSLLSQTAE QTRKLTDVET QVLNQTSRLE IQLLENSLST YKLEKQLLQQ TNEILKIHEK NSLLEHKILE MEGKHKEELD TLKEEKENLQ GLVSRQTFII Q
• Sequence Length: 498

• Applicable Applications for ANGPT1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Mus musculus (Mouse)
• Expression System: Prokaryotic expression
• Residues: Arg35~Gln231 (Accession # O08538) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 46048213
• NCBI GeneID: 11600
• NCBI Accession #: NP_033770.2
• NCBI GenBank Nucleotide #: NM_009640.4
• UniProt Accession #: O08538; Q6NWV7
• Molecular Weight: 24.2kDa
• NCBI Official Full Name: angiopoietin-1 isoform 1
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: Angpt1
• NCBI Official Synonym Symbols: Ang1; Ang-1; 1110046O21Rik
• NCBI Protein Information: angiopoietin-1
• UniProt Protein Name: Angiopoietin-1
• Protein Family: Angiopoietin
• UniProt Gene Name: Angpt1
• UniProt Synonym Gene Names: Agpt; ANG-1
• UniProt Entry Name: ANGP1_MOUSE

NCBI Description

This gene encodes a secreted glycoprotein that belongs to the angiopoietin family of vascular growth factors. The encoded protein is a ligand in the vascular tyrosine kinase signaling pathway and regulates the formation and stabilization of blood vessels. This protein also functions in striated muscles by promoting proliferation, migration and differentiation of skeletal myoblasts and plays an essential role in the vascular response to tissue injury. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]

Uniprot Description

ANGPT1: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: extracellular space; microvillus; plasma membrane; extracellular region; lipid raft

Molecular Function: receptor tyrosine kinase binding; vascular endothelial growth factor receptor binding; receptor binding

Biological Process: transmembrane receptor protein tyrosine kinase activation (dimerization); positive regulation of cell adhesion; multicellular organismal development; positive regulation of receptor internalization; negative regulation of protein import into nucleus; negative regulation of cell adhesion; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive chemotaxis; negative regulation of protein amino acid phosphorylation; ovarian follicle development; cardiac muscle morphogensis; hemopoiesis; negative regulation of neuron apoptosis; angiogenesis; regulation of I-kappaB kinase/NF-kappaB cascade; vasculogenesis; cell differentiation; protein homooligomerization; Tie receptor signaling pathway; in utero embryonic development; positive regulation of blood vessel endothelial cell migration; positive regulation of peptidyl-serine phosphorylation; cell-substrate adhesion; negative regulation of cytokine secretion during immune response; positive regulation of phosphoinositide 3-kinase cascade; patterning of blood vessels; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; heparin biosynthetic process; positive regulation of protein ubiquitination; regulation of protein binding; negative regulation of vascular permeability; regulation of tumor necrosis factor production; regulation of satellite cell proliferation; sprouting angiogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis; endoderm development