Typical Testing Data/Standard Curve (for reference only)
Human alpha-MSH ELISA Kit | AMSH elisa kit
Human alpha-MSH ELISA Kit
Gene Names
POMC; alpha-MSH
Reactivity
Human
Synonyms
alpha-MSH; Human alpha-MSH ELISA Kit; AMSH; alpha-melanotropin; AMSH elisa kit
Reactivity
Human
Specificity
This assay recognizes recombinant and natural human alpha-MSH. No significant cross-reactivity or interference was observed.
Sequence Length
49
Assay Type
Competitive
Detection Range
1.56-100 ng/mL
Sensitivity
0.6 ng/mL
Intra-Assay CV
<=4.2%
Inter-Assay CV
<=7.6%
Recovery
93%
Preparation and Storage
For long term storage, please store the entire kit at -20 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AMSH elisa kit
Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human alpha-msh,alpha-MSH concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to alpha-MSH, During the reaction, alpha-MSH in the sample or standard competes with a fixed amount of biotin-labeled alpha-MSH for sites on a pre-coated Monoclonal antibody specific to alpha-MSH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of alpha-MSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to alpha-MSH, During the reaction, alpha-MSH in the sample or standard competes with a fixed amount of biotin-labeled alpha-MSH for sites on a pre-coated Monoclonal antibody specific to alpha-MSH. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of alpha-MSH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
5,602 Da
NCBI Official Full Name
alpha-melanocyte-stimulating hormone, partial
NCBI Official Symbol
POMC
NCBI Official Synonym Symbols
alpha-MSH
NCBI Protein Information
proopiomelanocortin; alpha-melanocyte-stimulating hormone
UniProt Protein Name
Alpha-melanocyte-stimulating hormone
Protein Family
UniProt Gene Name
POMC
UniProt Entry Name
D2JZU1_CAPHI
Similar Products
Product Notes
The Human AMSH pomc (Catalog #AAA2884673) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2884673 ELISA Kit recognizes Human AMSH. It is sometimes possible for the material contained within the vial of "alpha-MSH, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
