Autocrine Motility Factor Receptor Recombinant Protein | AMFR recombinant protein

Recombinant Autocrine Motility Factor Receptor (AMFR)

Cat. Num. AAA2033123

• Gene Names: AMFR; GP78; RNF45
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Autocrine Motility Factor Receptor; Recombinant Autocrine Motility Factor Receptor (AMFR); GP78; RNF45; E3 ubiquitin-protein ligase AMFR; RING finger protein 45; AMFR recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: MAHQI QEMFPQVPYH LVLQDLQMTR SVEITTDNIL EGRIQVPFPTQRSDSLRPAL NSPVERPSPD LEEGEASVQT ERVPLDLSPR LEETLDFSEVELEPIEVEDF EARGSRFSKS ADERQRMLVQ RKDDLLQQAR KRFLNKSSEDDGASERLLPS EGTSSDPVTL RRRMLAAAAE RRLQRQRTT
• Sequence Length: 643

• Applicable Applications for AMFR recombinant protein: SDS-PAGE, Western (WB), ELISA (EIA), Immunoprecipitation (IP).
• Predicted Molecular Mass: 24.8kDa
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Endotoxin Level: <1.0EU per 1 ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Fragment: Met456~Thr639
• Organism Species: Mouse
• Tag: two N-terminal Tags, His-tag and T7-tag
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5931955
• NCBI GeneID: 267
• Molecular Weight: 72,996 Da
• NCBI Official Full Name: autocrine motility factor receptor
• NCBI Official Synonym Full Names: autocrine motility factor receptor, E3 ubiquitin protein ligase
• NCBI Official Symbol: AMFR
• NCBI Official Synonym Symbols: GP78; RNF45
• NCBI Protein Information: E3 ubiquitin-protein ligase AMFR
• UniProt Protein Name: E3 ubiquitin-protein ligase AMFR
• Protein Family: E3 ubiquitin-protein ligase
• UniProt Gene Name: AMFR
• UniProt Synonym Gene Names: RNF45; AMF receptor
• UniProt Entry Name: AMFR_HUMAN

NCBI Description

This locus encodes a glycosylated transmembrane receptor. Its ligand, autocrine motility factor, is a tumor motility-stimulating protein secreted by tumor cells. The encoded receptor is also a member of the E3 ubiquitin ligase family of proteins. It catalyzes ubiquitination and endoplasmic reticulum-associated degradation of specific proteins. [provided by RefSeq, Feb 2012]

Uniprot Description

AMFR: E3 ubiquitin-protein ligase that mediates the polyubiquitination of a number of proteins such as CD3D, CYP3A4, CFTR and APOB for proteasomal degradation. Component of a VCP/p97- AMFR/gp78 complex that participates in the final step of endoplasmic reticulum-associated degradation (ERAD). The VCP/p97- AMFR/gp78 complex is involved in the sterol-accelerated ERAD degradation of HMGCR through binding to the HMGCR-INSIG complex at the ER membrane and initiating ubiquitination of HMGCR. The ubiquitinated HMGCR is then released from the ER by the complex into the cytosol for subsequent destruction. Also acts as a scaffold protein to assemble a complex that couples ubiquitination, retranslocation and deglycosylation. Mediates tumor invasion and metastasis. Interacts with RNF5. Also forms an ERAD complex containing VCP/p97, NGLY1; PSMC1; SAKS1 AND RAD23B required for coupling retrotranslocation, ubiquitination and deglycosylation. Interacts with DRL1. Interacts (through a region distinct from the RING finger) with UBE2G2/UBC7. Component of the VCP/p97-AMFR/gp78 complex that enhances VCP/p97 binding to polyubiquitinated proteins for their degradation by the endoplasmic reticulum-associated degradation (ERAD) pathway. Interacts (via the VIM) with VCP/p97. Interacts (via its membrane domain) with INSIG1; the interaction initiates the sterol-mediated ubiquitination and degradation of HMGCR by the ERAD pathway. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Membrane protein, multi-pass; Ligase; EC 6.3.2.19; Ubiquitin conjugating system; EC 6.3.2.-; Endoplasmic reticulum; Receptor, misc.; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: cell soma; dendrite; endoplasmic reticulum membrane; growth cone; integral to endoplasmic reticulum membrane; integral to membrane; membrane; nucleus; perinuclear region of cytoplasm; protein complex

Molecular Function: chaperone binding; ligase activity; protein binding; protein binding, bridging; receptor activity; ubiquitin-protein ligase activity; zinc ion binding

Biological Process: aging; cell motility; ER-associated protein catabolic process; learning and/or memory; positive regulation of protein binding; protein autoubiquitination; protein oligomerization; protein polyubiquitination; protein ubiquitination during ubiquitin-dependent protein catabolic process; signal transduction; ubiquitin-dependent protein catabolic process; unfolded protein response