Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Rabbit anti-Rat Alanine Glyoxylate Aminotransferase Polyclonal Antibody | anti-AGXT antibody
Alanine Glyoxylate Aminotransferase (AGXT) Polyclonal Antibody
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-200
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Rat Liver Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant AGXT, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant AGXT, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Liver Tissue))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013]
Uniprot Description
AGXT: Defects in AGXT are the cause of hyperoxaluria primary type 1 (HP1); also known as primary hyperoxaluria type I (PH1) and oxalosis I. HP1 is a rare autosomal recessive inborn error of glyoxylate metabolism characterized by increased excretion of oxalate and glycolate, and the progressive accumulation of insoluble calcium oxalate in the kidney and urinary tract. Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - glycine, serine and threonine; EC 2.6.1.44; EC 2.6.1.51; Mitochondrial; Motility/polarity/chemotaxis; Transferase
Chromosomal Location of Human Ortholog: 9q36
Cellular Component: intracellular membrane-bound organelle; mitochondrial matrix; mitochondrion; peroxisomal matrix; peroxisome
Molecular Function: alanine-glyoxylate transaminase activity; amino acid binding; catalytic activity; protein homodimerization activity; protein self-association; pyridoxal phosphate binding; receptor binding; serine-pyruvate transaminase activity; transaminase activity
Biological Process: glycine biosynthetic process, by transamination of glyoxylate; glyoxylate catabolic process; glyoxylate metabolic process; L-alanine catabolic process; L-cysteine catabolic process; Notch signaling pathway; oxalic acid secretion; pyruvate biosynthetic process; response to cAMP; response to glucocorticoid stimulus
Research Articles on AGXT
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Product Notes
The AGXT agxt (Catalog #AAA2032254) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Alanine Glyoxylate Aminotransferase (AGXT) Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Alanine Glyoxylate Aminotransferase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western Blot: 1:50-400 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the AGXT agxt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alanine Glyoxylate Aminotransferase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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