Aldolase B Recombinant Protein | ALDOB recombinant protein

Recombinant Aldolase B, Fructose Bisphosphate (ALDOB)

Cat. Num. AAA2031189

• Gene Names: ALDOB; ALDB; ALDO2
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Aldolase B; Recombinant Aldolase B; Fructose Bisphosphate (ALDOB); ALDOB recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; IA QSIVANGKGI LAADESVGTM GNRLQRIKVENTEENRRQFR EILFSVDSSI NQSIGGVILF HETLYQKDSQ GKLFRNILKEKGIVVGIKLD QGGAPLAGTN KETTIQGLDG LSERCAQYKK DGVDFGKWRAVLRIADQCPS SLAIQENANA LARYASICQQ NGLVPIVEPE VIPDGDHDLEHCQYVTEKVL AAVYKALNDH HVYLEGTLLK PNMVTAGHAC TKKYTPEQVAMATVTALHRT VPAAVPGICF LSGGMSEEDA TLNLNAINLC PLPKPWKLSFSYGRALQASA LAAWGGKAAN KEATQEAFMK RAMANCQAAK GQYVHTGSSGAASTQSLFTA CYTY
• Sequence Length: 364

• Applicable Applications for ALDOB recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 40.6kDa
• Accurate Molecular Mass (KD): 41kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: two N-terminal tags, his-tag and T7 tag
• Organism Species: Homo sapiens (Human)
• Fragment: Ile9~Tyr364
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 2160383
• NCBI GeneID: 229
• Molecular Weight: 39,473 Da
• NCBI Official Full Name: aldolase B
• NCBI Official Synonym Full Names: aldolase B, fructose-bisphosphate
• NCBI Official Symbol: ALDOB
• NCBI Official Synonym Symbols: ALDB; ALDO2
• NCBI Protein Information: fructose-bisphosphate aldolase B
• UniProt Protein Name: Fructose-bisphosphate aldolase B
• Protein Family: Fructose-bisphosphate aldolase
• UniProt Gene Name: ALDOB
• UniProt Synonym Gene Names: ALDB
• UniProt Entry Name: ALDOB_HUMAN

NCBI Description

Fructose-1,6-bisphosphate aldolase (EC 4.1.2.13) is a tetrameric glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Vertebrates have 3 aldolase isozymes which are distinguished by their electrophoretic and catalytic properties. Differences indicate that aldolases A, B, and C are distinct proteins, the products of a family of related 'housekeeping' genes exhibiting developmentally regulated expression of the different isozymes. The developing embryo produces aldolase A, which is produced in even greater amounts in adult muscle where it can be as much as 5% of total cellular protein. In adult liver, kidney and intestine, aldolase A expression is repressed and aldolase B is produced. In brain and other nervous tissue, aldolase A and C are expressed about equally. There is a high degree of homology between aldolase A and C. Defects in ALDOB cause hereditary fructose intolerance. [provided by RefSeq, Dec 2008]

Uniprot Description

ALDOB: Defects in ALDOB are the cause of hereditary fructose intolerance (HFI). HFI is an autosomal recessive disease that results in an inability to metabolize fructose and related sugars. Complete exclusion of fructose results in dramatic recovery; however, if not treated properly, HFI subjects suffer episodes of hypoglycemia, general ill condition, and risk of death the remainder of life. Belongs to the class I fructose-bisphosphate aldolase family.

Protein type: Lyase; Carbohydrate Metabolism - pentose phosphate pathway; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - fructose and mannose; EC 4.1.2.13

Chromosomal Location of Human Ortholog: 9q21.3-q22.2

Cellular Component: microtubule organizing center; cytosol

Molecular Function: identical protein binding; protein binding; cytoskeletal protein binding; fructose-bisphosphate aldolase activity; ATPase binding

Biological Process: fructose 1,6-bisphosphate metabolic process; glycolysis; NADH oxidation; positive regulation of ATPase activity; carbohydrate metabolic process; glucose metabolic process; pathogenesis; fructose catabolic process; fructose metabolic process; gluconeogenesis

Disease: Fructose Intolerance, Hereditary