Human Aldehyde Dehydrogenase, Mitochondrial ELISA Kit | ALDH elisa kit

Human Aldehyde Dehydrogenase, Mitochondrial ELISA Kit

Cat. Num. AAA760201

• Reactivity: Human
• Synonyms: Aldehyde Dehydrogenase; Mitochondrial; Human Aldehyde Dehydrogenase; Mitochondrial ELISA Kit; ALDH/ALDH2 (Aldehyde dehydrogenase; mitochondrial)/ALDHI/ALDH class 2/ALDH-E2/ALDM/Aldehyde Dehydrogenase 2; ALDH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ALDH. No significant cross-reactivity or interference between ALDH and analogues was observed.
• Sequence Length: 519

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ALDH elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 14192933
• NCBI GeneID: 29539
• NCBI Accession #: NP_115792.1
• NCBI GenBank Nucleotide #: NM_032416.1
• Molecular Weight: 56,488 Da
• NCBI Official Full Name: aldehyde dehydrogenase, mitochondrial
• NCBI Official Synonym Full Names: aldehyde dehydrogenase 2 family (mitochondrial)
• NCBI Official Symbol: Aldh2
• NCBI Protein Information: aldehyde dehydrogenase, mitochondrial
• UniProt Protein Name: Aldehyde dehydrogenase, mitochondrial
• Protein Family: NADP-dependent fatty aldehyde dehydrogenase
• UniProt Gene Name: Aldh2
• UniProt Entry Name: ALDH2_RAT

Uniprot Description

ALDH2: This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Mar 2011]

Protein type: Amino Acid Metabolism - arginine and proline; Secondary Metabolites Metabolism - limonene and pinene degradation; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - propanoate; Oxidoreductase; EC 1.2.1.3; Other Amino Acids Metabolism - beta-alanine; Carbohydrate Metabolism - pyruvate; Mitochondrial; Amino Acid Metabolism - lysine degradation; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - valine, leucine and isoleucine degradation; Amino Acid Metabolism - histidine; Lipid Metabolism - glycerolipid; Lipid Metabolism - fatty acid; Carbohydrate Metabolism - butanoate

Cellular Component: mitochondrial matrix; mitochondrion

Molecular Function: aldehyde dehydrogenase (NAD) activity; identical protein binding; oxidoreductase activity, acting on the aldehyde or oxo group of donors, NAD or NADP as acceptor; protein binding

Biological Process: cellular response to hormone stimulus; ethanol catabolic process; liver development; negative regulation of apoptosis; response to estradiol stimulus; response to hyperoxia; response to lipopolysaccharide; response to nicotine; response to progesterone stimulus; response to testosterone stimulus