AKR1C4 recombinant protein

Recombinant Human AKR1C4 Protein

Cat. Num. AAA8248524

• Gene Names: AKR1C4; C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• Reactivity: Human
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: AKR1C4; Recombinant Human AKR1C4 Protein; CHDR; Aldo-keto reductase family 1 member C4; 3-alpha-HSD1; 3-alpha-hydroxysteroid dehydrogenase type I; Chlordecone reductase; CDR; Dihydrodiol dehydrogenase 4; DD-4; DD4; HAKRA; AKR1C4 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Supplied as a 0.2 muM filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.
• Sequence: MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNY RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY
• Sequence Length: 323

• Applicable Applications for AKR1C4 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for AKR1C4 recombinant protein

Recombinant Human AKR1C4 Protein is produced by our E Coli expression system and the target gene encoding Met1-Tyr323 is expressed with a 6His tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 325652083
• NCBI GeneID: 1109
• NCBI Accession #: NP_001809.3
• NCBI GenBank Nucleotide #: NM_001818.3
• UniProt Accession #: P17516; Q5T6A3; Q8WW84; Q9NS54; AAB47003
• Molecular Weight: 37,067 Da
• NCBI Official Full Name: aldo-keto reductase family 1 member C4
• NCBI Official Synonym Full Names: aldo-keto reductase family 1 member C4
• NCBI Official Symbol: AKR1C4
• NCBI Official Synonym Symbols: C11; CDR; DD4; CHDR; DD-4; HAKRA; 3-alpha-HSD
• NCBI Protein Information: aldo-keto reductase family 1 member C4
• UniProt Protein Name: Aldo-keto reductase family 1 member C4
• Protein Family: Aldo-keto reductase family
• UniProt Gene Name: AKR1C4
• UniProt Synonym Gene Names: CHDR; CDR; DD-4; DD4
• UniProt Entry Name: AK1C4_HUMAN

NCBI Description

This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. This gene shares high sequence identity with three other gene members and is clustered with those three genes at chromosome 10p15-p14. [provided by RefSeq, Jul 2008]

Uniprot Description

AKR1C4: Catalyzes the transformation of the potent androgen dihydrotestosterone (DHT) into the less active form, 5-alpha- androstan-3-alpha,17-beta-diol (3-alpha-diol). Also has some 20- alpha-hydroxysteroid dehydrogenase activity. The biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leads to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. Defects in AKR1C4 are a cause of 46,XY sex reversal type 8 (SRXY8). A disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. AKR1C4 mutations may act as modifier of disease severity in SRXY8 patients. A splicing mutation resulting in loss of exon 2 has been found in affected individuals also carrying mutation Val-79 in AKR1C2 (PubMed:21802064). Belongs to the aldo/keto reductase family.

Protein type: EC 1.1.1.225; EC 1.1.1.357; Lipid Metabolism - C21-steroid hormone; Lipid Metabolism - androgen and estrogen; Lipid Metabolism - primary bile acid biosynthesis; Oxidoreductase; Xenobiotic Metabolism - metabolism by cytochrome P450

Chromosomal Location of Human Ortholog: 10p15.1

Cellular Component: cytoplasm; cytosol

Molecular Function: 3(or 17)-alpha-hydroxysteroid dehydrogenase activity; aldo-keto reductase activity; bile acid transmembrane transporter activity; electron carrier activity; oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor; retinal dehydrogenase activity

Biological Process: androgen metabolic process; bile acid and bile salt transport; bile acid biosynthetic process; retinoid metabolic process; steroid metabolic process

Disease: 46,xy Sex Reversal 8