Allograft Inflammatory Factor 1 Recombinant Protein | AIF1 recombinant protein

Recombinant Human Allograft Inflammatory Factor 1

Cat. Num. AAA143756

• Gene Names: AIF1; IBA1; IRT1; AIF-1; IRT-1
• Purity: Greater than 90% as determined by SDS PAGE.
• Synonyms: Allograft Inflammatory Factor 1; Recombinant Human Allograft Inflammatory Factor 1; AIF1 Human; Allograft Inflammatory Factor 1 Human Recombinant; AIF-1; Allograft inflammatory factor 1; Em:AF129756.17; G1; IBA1; Ionized calcium-binding adapter molecule 1; IRT-1; Protein G1; AIF1; AIF1 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90% as determined by SDS PAGE.
• Form/Format: Filtered and lyophilized from 0.5mg/ml in 20mM Tris buffer and 50mM NaCl pH-7.5.; Filtered White lyophilized (freeze-dried) powder.
• Sequence: MKHHHHHHASQTRDLQGGKAF­GLLKAQQEERLDE­INKQFLDDPKYSSDED­LPSKLEGFKEKYMEF­DLNGNGDIDIMSLKRMLEK­LGVPKTHLELKKLI­GEVSSGSGETFSYP­DFLRMMLGKRSAIL­KMILMYEEKAREKEK­PTGPPAKKAISELP
• Sequence Length: 147

• Solubility: Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely.
• Preparation and Storage: For long term, store lyophilized AIF1 at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.The lyophilized protein remains stable for 24 months when stored at -20 degree C.

Related Product Information for AIF1 recombinant protein

Description: The AIF1 Human Recombinant contains a total of 155 amino acids having a molecular Mass of 17.7kDa. The Human AIF1 is fused to a 9 amino acid long N-terminal His tag.

Introduction: Human AIF1 protein shares 98% homology/identity with that of rat. AIF1 is expressed in macrophages and neutrophils. The expression of AIF1 transcripts is upregulated by IFN-g in rat macrophages. AIF1 is expressed selectively in human macrophage-like cell lines, and in a subset of CD68(+) macrophages in the interstitial and perivascular spaces of human heart allografts. In quiescent cultured human vascular smooth muscle cells synthesis of AIF1 is induced by IFN-g, IL1b, and conditioned medium of T-cells. Overexpression of AIF1 in human VSMCs results in enhanced growth of these cells. AIF1 is expressed during apoptosis rat mammary gland and ventral prostate tissues. Allograft Inflammatory Factor 1 is expressed by several tumor-associated activated macrophages and microglial cells in rat and human gliomas. There is an evident relationship of AIF1-expressing activated macrophages and microglial cells with tumor malignancy in humans.

Product Categories/Family for AIF1 recombinant protein

Cytokines and Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 14574568
• NCBI GeneID: 199
• NCBI Accession #: NP_001614.3
• NCBI GenBank Nucleotide #: NM_001623.3
• UniProt Accession #: P55008; O43904; Q9UIV4; Q9UKS9; A8K406
• Molecular Weight: 14,617 Da
• NCBI Official Full Name: allograft inflammatory factor 1 isoform 3
• NCBI Official Synonym Full Names: allograft inflammatory factor 1
• NCBI Official Symbol: AIF1
• NCBI Official Synonym Symbols: IBA1; IRT1; AIF-1; IRT-1
• NCBI Protein Information: allograft inflammatory factor 1; allograft inflammatory factor-1 splice variant Hara-1; interferon gamma responsive transcript; ionized calcium-binding adapter molecule 1; protein G1
• UniProt Protein Name: Allograft inflammatory factor 1
• Protein Family: Apoptosis-inducing factor
• UniProt Gene Name: AIF1
• UniProt Synonym Gene Names: G1; IBA1; AIF-1
• UniProt Entry Name: AIF1_HUMAN

NCBI Description

This gene is induced by cytokines and interferon. Its protein product is thought to be involved in negative regulation of growth of vascular smooth muscle cells, which contributes to the anti-inflammatory response to vessel wall trauma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Uniprot Description

AIF1: Actin-binding protein that enhances membrane ruffling and RAC activation. Enhances the actin-bundling activity of LCP1. Binds calcium. Plays a role in RAC signaling and in phagocytosis. May play a role in macrophage activation and function. Promotes the proliferation of vascular smooth muscle cells and of T- lymphocytes. Enhances lymphocyte migration. Plays a role in vascular inflammation. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: perinuclear region of cytoplasm; lamellipodium; cytoplasm; actin filament; perikaryon; nucleus; cytosol; phagocytic cup

Molecular Function: actin filament binding; calcium ion binding

Biological Process: actin filament bundle formation; positive regulation of nitric oxide biosynthetic process; negative regulation of smooth muscle cell proliferation; positive regulation of smooth muscle cell proliferation; Rac protein signal transduction; microglial cell activation; cellular response to hormone stimulus; actin filament polymerization; positive regulation of muscle hyperplasia; cellular response to extracellular stimulus; positive regulation of T cell proliferation; response to axon injury; positive regulation of protein amino acid phosphorylation; phagocytosis, engulfment; inflammatory response; response to electrical stimulus; negative regulation of apoptosis