Human Apoptosis Inducing Factor ELISA Kit | AIF elisa kit

Human Apoptosis Inducing Factor ELISA Kit

Cat. Num. AAA760190

• Gene Names: AIF; aif; CG7263; DmAIF; DmelCG7263; SD03428p
• Reactivity: Human
• Synonyms: Apoptosis Inducing Factor; Human Apoptosis Inducing Factor ELISA Kit; AIF/AIFM1/COXPD6/PDCD8/AIFapoptosis-inducing factor 1; mitochondrial/apoptosis-inducing factor; mitochondrion-associated; 1/COXPD6/MGC111425/PDCD8striatal apoptosis-inducing factor/programmed cell death 8 (apoptosis-inducing factor)/Programmed cell death protein 8; AIF elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of AIF. No significant cross-reactivity or interference between AIF and analogues was observed.
• Sequence Length: 738

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AIF elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 442625344
• NCBI GeneID: 33390
• NCBI Accession #: NP_001259907.1
• NCBI GenBank Nucleotide #: NM_001272978.1
• Molecular Weight: 74,050 Da
• NCBI Official Full Name: apoptosis inducing factor, isoform C
• NCBI Official Synonym Full Names: Apoptosis inducing factor
• NCBI Official Symbol: AIF
• NCBI Official Synonym Symbols: aif; CG7263; DmAIF; DmelCG7263; SD03428p
• NCBI Protein Information: CG7263 gene product from transcript CG7263-RC
• UniProt Protein Name: Putative apoptosis-inducing factor 1, mitochondrial
• Protein Family: Putative apoptosis-inducing factor
• UniProt Gene Name: AIF
• UniProt Synonym Gene Names: DmAIF
• UniProt Entry Name: AIFM1_DROME

Uniprot Description

Probable NADH oxidoreductase (). Mitochondrial effector of cell death that plays roles in developmentally regulated cell death and normal mitochondrial function.