SDS-Page
AHSP recombinant protein
AHSP Protein, Human, Recombinant
Gene Names
AHSP; EDRF; ERAF
Purity
>90% as determined by SDS-PAGE
Synonyms
AHSP; AHSP Protein; Human; Recombinant; Human AHSP/ERAF Protein; EDRF Protein; ERAF Protein; alpha hemoglobin stabilizing protein; AHSP recombinant protein
Host
E Coli
Purity/Purification
>90% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Ser102
Species
Human
Predicted N Terminal
Met
Tag
Native
Protein Construction
A DNA sequence encoding human ERAF (Q9NZD4)(Met1-Ser102) was expressed.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for AHSP recombinant protein
Background: AHSP, also known as ERAF, is a conserved mammalian erythroid protein which belongs to the AHSP family. It is expressed in blood and bone marrow. AHSP facilitates the production of Hemoglobin A by stabilizing free alpha-globin. It rapidly binds to ferrous alpha with association (k'(AHSP)) and dissociation (k(AHSP)) rate constants of ~1 um(-1) s(-1) and.2 s(-1), respectively, at pH 7.4 at 22 degree C. A small slow phase was observed when AHSP binds to excess ferrous alphaCO. This slow phase appears to be due to cis to trans prolyl isomerization of the Asp(29)-Pro(3) peptide bond in wild-type AHSP because it was absent when alphaCO was mixed with P3A and P3W AHSP, which are fixed in the trans conformation. This slow phase was also absent when met(Fe(3+))-alpha reacted with wild-type AHSP, suggesting that met-alpha is capable of rapidly binding to either Pro(3) conformer. Both wild-type and Pro(3)-substituted AHSPs drive the formation of a met-alpha hemichrome conformation following binding to either met- or oxy(Fe(2+))-alpha. The dissociation rate of the met-alpha-AHSP complex (k(AHSP) ~.2 s(-1)) is ~1-fold slower than that for ferrous alpha-AHSP complexes, resulting in a much higher affinity of AHSP for met-alpha. Thus, in vivo, AHSP acts as a molecular chaperone by rapidly binding and stabilizing met-alpha hemichrome folding intermediates. The low rate of met-alpha dissociation also allows AHSP to have a quality control function by kinetically trapping ferric alpha and preventing its incorporation into less stable mixed valence Hemoglobin A tetramers. Reduction of AHSP-bound met-alpha allows more rapid release to beta subunits to form stable fully, reduced hemoglobin dimers and tetramers.
References
Miele G., et al.,(2001), A novel erythroid-specific marker of transmissible spongiform encephalopathies. Nat. Med. 7:361-364.Zhang Q.-H., et al., (2000), Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.Genome Res. 10:1546-1560.Kihm A.J., et al.,(2002), An abundant erythroid protein that stabilizes free alpha-haemoglobin.Nature 417:758-763.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
11,840 Da
NCBI Official Full Name
alpha-hemoglobin-stabilizing protein
NCBI Official Synonym Full Names
alpha hemoglobin stabilizing protein
NCBI Official Symbol
AHSP
NCBI Official Synonym Symbols
EDRF; ERAF
NCBI Protein Information
alpha-hemoglobin-stabilizing protein; alpha hemoglobin stabilising protein; erythroid associated factor; erythroid differentiation associated factor; erythroid differentiation-related factor; erythroid-associated factor
UniProt Protein Name
Alpha-hemoglobin-stabilizing protein
Protein Family
UniProt Gene Name
AHSP
UniProt Synonym Gene Names
EDRF; ERAF
UniProt Entry Name
AHSP_HUMAN
Uniprot Description
AHSP: Acts as a chaperone to prevent the harmful aggregation of alpha-hemoglobin during normal erythroid cell development. Specifically protects free alpha-hemoglobin from precipitation. It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Belongs to the AHSP family.
Chromosomal Location of Human Ortholog: 16p11.2
Cellular Component: hemoglobin complex
Molecular Function: hemoglobin binding; unfolded protein binding
Biological Process: protein folding; protein stabilization; hemoglobin metabolic process; hemopoiesis
Research Articles on AHSP
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Product Notes
The AHSP ahsp (Catalog #AAA8121680) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Ser10 2. It is sometimes possible for the material contained within the vial of "AHSP, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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