Typical Testing Data/Standard Curve (for reference only)
Human Angiotensin 2 Receptor type 2 Autoantibodies ELISA Kit | AGTR2 AA elisa kit
Human Angiotensin 2 Receptor type 2 Autoantibodies ELISA Kit
Gene Names
Nat2; Nat2a
Reactivity
Human
Synonyms
Angiotensin 2 Receptor type 2 Autoantibodies; Human Angiotensin 2 Receptor type 2 Autoantibodies ELISA Kit; AGTR2 AA elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This AT2R-AA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human AT2R-AA. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AGTR2 AA elisa kit
Principle of the assay: AT2R-AA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AT2R-AA antibody and an AT2R-AA-HRP conjugate. The assay sample and buffer are incubated together with AT2R-AA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AT2R-AA concentration since AT2R-AA from samples and AT2R-AA-HRP conjugate compete for the anti-AT2R-AA antibody binding site. Since the number of sites is limited, as more sites are occupied by AT2R-AA from the sample, fewer sites are left to bind AT2R-AA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AT2R-AA concentration in each sample is interpolated from this standard curve.
Product Categories/Family for AGTR2 AA elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,756 Da
NCBI Official Full Name
arylamine N-acetyltransferase 2
NCBI Official Synonym Full Names
N-acetyltransferase 2 (arylamine N-acetyltransferase)
NCBI Official Symbol
Nat2
NCBI Official Synonym Symbols
Nat2a
NCBI Protein Information
arylamine N-acetyltransferase 2; AT-2; NAT-2; arylamide acetylase 2; N-acetyltransferase type 2; N-Acetyltransferase-2 (arylamine N-acetyltransferase)
UniProt Protein Name
Arylamine N-acetyltransferase 2
UniProt Gene Name
Nat2
UniProt Synonym Gene Names
Aac2; AT-2; NAT-2
UniProt Entry Name
ARY2_RAT
NCBI Description
enzyme that acetylates only aryl amines; may have a role in carcinogenesis [RGD, Feb 2006]
Uniprot Description
NAT1: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Belongs to the arylamine N-acetyltransferase family.
Protein type: Secondary Metabolites Metabolism - caffeine; EC 2.3.1.5; Acetyltransferase; Xenobiotic Metabolism - drug metabolism - other enzymes
Cellular Component: cytosol
Molecular Function: N-acetyltransferase activity; arylamine N-acetyltransferase activity
Biological Process: response to xenobiotic stimulus; gut development; metabolic process; response to lipopolysaccharide
Research Articles on AGTR2 AA
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Product Notes
The Human AGTR2 AA nat2 (Catalog #AAA724547) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA724547 ELISA Kit recognizes Human AGTR2 AA. It is sometimes possible for the material contained within the vial of "Angiotensin 2 Receptor type 2 Autoantibodies, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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