Arylformamidase Recombinant Protein | AFMID recombinant protein
Recombinant Arylformamidase (AFMID)
Gene Names
AFMID; KF; FKF; KFA
Applications
SDS-Page, Western Blot
Purity
>80%
Synonyms
Arylformamidase; Recombinant Arylformamidase (AFMID); KF; KFA; FKF; Kynurenine formamidase; N-formylkynurenine formamidase; AFMID recombinant protein
Host
E Coli
Purity/Purification
>80%
Form/Format
Freeze-dried powder
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Sequence Positions
Met1~Gln303
Sequence Length
303
Applicable Applications for AFMID recombinant protein
Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
Source
Prokaryotic expression
Species
Human
Tag
N-terminal His and GST Tag
Subcellular Location
Nucleus, Cytoplasm
Isoelectric Point
5.8
Reconstitution
Reconstitute in PBS or others.
Preparation and Storage
Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months. Avoid repeated freeze/thaw cycles.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Product Categories/Family for AFMID recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Mass: 64kDa
Accurate Molecular Mass: 54kDa
Accurate Molecular Mass: 54kDa
NCBI Official Full Name
kynurenine formamidase isoform 2
NCBI Official Synonym Full Names
arylformamidase
NCBI Official Symbol
AFMID
NCBI Official Synonym Symbols
KF; FKF; KFA
NCBI Protein Information
kynurenine formamidase
UniProt Protein Name
Kynurenine formamidase
UniProt Gene Name
AFMID
UniProt Synonym Gene Names
KFA; KFase; FKF
Uniprot Description
AFMID: Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites. Belongs to the kynurenine formamidase family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Amino Acid Metabolism - tryptophan; Carbohydrate Metabolism - glyoxylate and dicarboxylate; EC 3.5.1.9; Hydrolase
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoplasm; cytosol; nucleus
Molecular Function: arylformamidase activity; hydrolase activity
Biological Process: tryptophan catabolic process to kynurenine
Research Articles on AFMID
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Product Notes
The AFMID afmid (Catalog #AAA2123889) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is Met1~Gln303. AAA Biotech's Arylformamidase can be used in a range of immunoassay formats including, but not limited to, Positive Control, Immunogen, SDS-PAGE, Western Blot (WB). Researchers should empirically determine the suitability of the AFMID afmid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Arylformamidase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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