Rat Actin filament-associated protein 1 ELISA Kit | AFAP1 elisa kit
Rat Actin filament-associated protein 1 (AFAP1) ELISA Kit
Gene Names
Afap1; Afap
Reactivity
Rat
Synonyms
Actin filament-associated protein 1; Rat Actin filament-associated protein 1 (AFAP1) ELISA Kit; AFAP1 elisa kit
Reactivity
Rat
Specificity
This assay has high sensitivity and excellent specificity for detection of Rat AFAP1. No significant cross-reactivity or interference between Rat AFAP1 and analogues was observed.
Samples
Serum, Plasma, Other biological fluids
Assay Type
Sandwich
Sample Volume
1-200 uL
Precision
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV
Inter-Assay: CV
Detection Wavelength
450 nm
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for AFAP1 elisa kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate AFAP1 in samples. An antibody specific for AFAP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyAFAP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AFAP1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AFAP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
80,752 Da
NCBI Official Full Name
actin filament-associated protein 1
NCBI Official Synonym Full Names
actin filament associated protein 1
NCBI Official Symbol
Afap1
NCBI Official Synonym Symbols
Afap
NCBI Protein Information
actin filament-associated protein 1
UniProt Protein Name
Actin filament-associated protein 1
Protein Family
UniProt Gene Name
Afap1
UniProt Synonym Gene Names
Afap; AFAP-110
Uniprot Description
AFAP: Can cross-link actin filaments into both network and bundle structures. May modulate changes in actin filament integrity and induce lamellipodia formation. May function as an adapter molecule that links other proteins, such as SRC and PKC to the actin cytoskeleton. Seems to play a role in the development and progression of prostate adenocarcinoma by regulating cell-matrix adhesions and migration in the cancer cells.
Protein type: Adaptor/scaffold
Chromosomal Location of Human Ortholog: 14q21
Cellular Component: actin cytoskeleton; actin filament; cytoplasm; cytosol; focal adhesion
Molecular Function: actin binding; protein binding; SH2 domain binding; SH3 domain binding
Biological Process: regulation of cytoskeleton organization; regulation of signal transduction; signal transduction
Research Articles on AFAP1
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Product Notes
The Rat AFAP1 afap1 (Catalog #AAA283183) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283183 ELISA Kit recognizes Rat AFAP1. It is sometimes possible for the material contained within the vial of "Actin filament-associated protein 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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