NADP-dependent alkenal double bond reductase P1 (P1) Recombinant Protein | P1 recombinant protein

Recombinant Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1 (P1)

Cat. Num. AAA9423610

• Gene Names: AER; alkenal reductase; AT-AER; F2K13.120; F2K13_120
• Reactivity: Mouse-ear cress
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: NADP-dependent alkenal double bond reductase P1 (P1); Recombinant Arabidopsis thaliana NADP-dependent alkenal double bond reductase P1 (P1); DBR1; P1 recombinant protein

• Host: E Coli
• Reactivity: Mouse-ear cress
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: Tris-based buffer, 50% glycerol
• Sequence Positions: Full Length, 1-345aa

• Tag Info: N-terminal 6xHis-SUMO-tagged
• Preparation and Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.; Generally, the shelf life of liquid form is 6 months at -20 degree C/-80 degree C. The shelf life of lyophilized form is 12 months at -20 degree C/-80 degree C.

Related Product Information for P1 recombinant protein

Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro). Has activity towards toxic substrates, such as 4-hydroxy- (2E)-nonenal (in vitro). May play a distinct role in plant antioxidant defense and is possibly involved in NAD (P)/NAD (P)H homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 15237888
• NCBI GeneID: 831560
• NCBI Accession #: NP_197199.1
• NCBI GenBank Nucleotide #: NM_121703.4
• UniProt Accession #: Q39172; Q501A9; Q8L865
• Molecular Weight: 53.95 kDa
• NCBI Official Full Name: alkenal reductase
• NCBI Official Symbol: AER
• NCBI Official Synonym Symbols: alkenal reductase; AT-AER; F2K13.120; F2K13_120
• NCBI Protein Information: alkenal reductase
• UniProt Protein Name: NADPH-dependent oxidoreductase 2-alkenal reductase
• Protein Family: NADP-dependent alkenal double bond reductase
• UniProt Gene Name: AER
• UniProt Synonym Gene Names: P1-ZCr

NCBI Description

encodes a 2-alkenal reductase (EC 1.3.1.74), plays a key role in the detoxification of reactive carbonyls

Uniprot Description

Involved in the detoxification of reactive carbonyls (PubMed:10848984, PubMed:12514241, PubMed:16299173). Acts on lipid peroxide-derived reactive aldehydes (PubMed:12514241). Specific to a double bond activated by an adjacent carbonyl group (PubMed:12514241). Can use both quinones and diamide as substrates, but not menadione, ferricyanide or phylloquinone (PubMed:10848984). Can use 4-hydroxy-(2E)-nonenal (HNE), 4-hydroxy-(2E)-hexenal (HHE), (2E)-nonenal, (2E)-hexenal, (2E)-pentenal, propenal (acrolein), 3-buten-2-one and 3-penten-2-one, but not (R)---carvone, n-nonanal, n-hexanal, (3Z)-hexanal, cyclohex-2-en-1-one or 12-oxo phytodienoic acid (OPDA) as electron acceptors (PubMed:12514241). Catalyzes the reduction of the alpha,beta-unsaturated bond of 2-alkenals, of lipid peroxide-derived oxenes 9-oxo-10(E),12(Z)-octadecadienoic acid (9-KODE) and 13-oxo-9(Z),11(E)-octadecadienoic acid (13-KODE), as well as 4-oxo-(2E)-nonenal and 4-hydroxynonenal (PubMed:16299173). Can use 12-oxo-10(E) dodecanoate (traumatin), trans-1,3 diphenyl-2-propenone, trans-1,4-diphenyl-2-butene-1,4-dione, 9-oxo-12,13-epoxy-(10E)-octadecenoic acid (trans-EKODE-1b) and 9,13-dihydroxy-10-oxo-11-octadecenoic acid as substrates (PubMed:26678323). Catalyzes the reduction of the 7-8 double bond of phenylpropanal substrates, such as p-coumaryl aldehyde and coniferyl aldehyde (in vitro) (PubMed:17028190). Has activity towards toxic substrates, such as 4-hydroxy-(2E)-nonenal (in vitro) (PubMed:17028190). May play a distinct role in plant antioxidant defense and is possibly involved in NAD(P)/NAD(P)H homeostasis (PubMed:17028190).