Beta-1 adrenergic receptor (ADRB1) Recombinant Protein | ADRB1 recombinant protein

Recombinant Human Beta-1 adrenergic receptor (ADRB1)

Cat. Num. AAA7137049

• Gene Names: ADRB1; RHR; B1AR; ADRB1R; BETA1AR
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Beta-1 adrenergic receptor (ADRB1); Recombinant Human Beta-1 adrenergic receptor (ADRB1); Beta-1 adrenoreceptor; ADRB1 recombinant protein

• Host: Cell Free Expression
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Liquid containing glycerol
• Sequence Positions: 1-477aa, Full Length
• Sequence: MGAGVLVLGASEPGNLSSAAPLPDGAATAARLLVPASPPASLLPPASESPEPLSQQWTAGMGLLMALIVLLIVAGNVLVIVAIAKTPRLQTLTNLFIMSLASADLVMGLLVVPFGATIVVWGRWEYGSFFCELWTSVDVLCVTASIETLCVIALDRYLAITSPFRYQSLLTRARARGLVCTVWAISALVSFLPILMHWWRAESDEARRCYNDPKCCDFVTNRAYAIASSVVSFYVPLCIMAFVYLRVFREAQKQVKKIDSCERRFLGGPARPPSPSPSPVPAPAPPPGPPRPAAAAATAPLANGRAGKRRPSRLVALREQKALKTLGIIMGVFTLCWLPFFLANVVKAFHRELVPDRLFVFFNWLGYANSAFNPIIYCRSPDFRKAFQRLLCCARRAARRRHATHGDRPRASGCLARPGPPPSPGAASDDDDDDVVGATPPARLLEPWAGCNGGAAADSDSSLDEPCRPGFASESKV

• Application Notes: This is a recombinant transmembrane protein expressed in a cell-free expression system.
• Species: Human
• Tag: N-terminal 10xHis-tagged
• Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Mediates Ras activation through G (s) -alpha- and cAMP-mediated signaling. Involved in the regulation of sleep/wake behaviors (PubMed:31473062).
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL. We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 degree C/-80 degree C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.; Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Product Categories/Family for ADRB1 recombinant protein

Cardiovascular

References

"Association between obesity and a polymorphism in the beta (1) -adrenoceptor gene (Gly389Arg ADRB1) in Caucasian women."Dionne I.J., Garant M.J., Nolan A.A., Pollin T.I., Lewis D.G., Shuldiner A.R., Poehlman E.T.Int. J. Obes. Relat. Metab. Disord. 26:633-639 (2002)

NCBI and Uniprot Product Information

• NCBI GI #: 4557265
• NCBI GeneID: 153
• NCBI Accession #: NP_000675.1
• NCBI GenBank Nucleotide #: NM_000684.2
• UniProt Accession #: P08588; Q5T5Y4; Q9UKG7; Q9UKG8; B0LPE2
• NCBI Official Full Name: beta-1 adrenergic receptor
• NCBI Official Synonym Full Names: adrenoceptor beta 1
• NCBI Official Symbol: ADRB1
• NCBI Official Synonym Symbols: RHR; B1AR; ADRB1R; BETA1AR
• NCBI Protein Information: beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; adrenergic, beta-1-, receptor
• UniProt Protein Name: Beta-1 adrenergic receptor
• Protein Family: Beta-1 adrenergic receptor
• UniProt Gene Name: ADRB1
• UniProt Synonym Gene Names: ADRB1R; B1AR; Beta-1 adrenoceptor
• UniProt Entry Name: ADRB1_HUMAN

NCBI Description

The adrenergic receptors (subtypes alpha 1, alpha 2, beta 1, and beta 2) are a prototypic family of guanine nucleotide binding regulatory protein-coupled receptors that mediate the physiological effects of the hormone epinephrine and the neurotransmitter norepinephrine. Specific polymorphisms in this gene have been shown to affect the resting heart rate and can be involved in heart failure. [provided by RefSeq, Jul 2008]

Uniprot Description

ADRB1: a GPCR-coupled 7 transmembrane receptor that binds epinephrine and norepinephrine with approximately equal affinity. Mediates the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Interacts with PIST and PSD-95. Localized at the plasma membrane and in the Golgi upon PIST overexpression. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase.

Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 10q25.3

Cellular Component: integral to plasma membrane; early endosome; plasma membrane; nucleus

Molecular Function: protein binding; protein heterodimerization activity; receptor signaling protein activity; dopamine binding; drug binding; norepinephrine binding; epinephrine binding; beta-adrenergic receptor activity; alpha-2A adrenergic receptor binding; Ras guanyl-nucleotide exchange factor activity; beta1-adrenergic receptor activity; PDZ domain binding

Biological Process: fear response; diet induced thermogenesis; wound healing; positive regulation of apoptosis; apoptosis; lipid homeostasis; positive regulation of systemic arterial blood pressure; sensory perception of pain; positive regulation of saliva secretion; Rho protein signal transduction; regulation of inhibitory postsynaptic membrane potential; negative regulation of smooth muscle contraction; positive regulation of cAMP biosynthetic process; aging; glycogen catabolic process; negative regulation of multicellular organism growth; adenylate cyclase activation; regulation of calcium ion transport; memory; positive regulation of the force of heart contraction by norepinephrine; protein localization in organelle; heat generation; brown fat cell differentiation; response to cold; positive regulation of heart rate by epinephrine-norepinephrine; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure

Disease: Resting Heart Rate, Variation In