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SDS-Page

Adenylate Cyclase 9 (ADCY9) Recombinant Protein | ADCY9 recombinant protein

Recombinant Adenylate Cyclase 9 (ADCY9)

Gene Names
ADCY9; AC9
Applications
SDS-Page, Western Blot, ELISA, Immunoprecipitation
Purity
> 95%
Synonyms
Adenylate Cyclase 9 (ADCY9); Recombinant Adenylate Cyclase 9 (ADCY9); ADCY9 recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
> 95%
Form/Format
Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Sequence
The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DW LLRNIIPYHV AEQLKVSQTY SKNHDSGGVI FASIVNFSEF YEENYEGGKE CYRVLNELIG DFDELLSKPD YSSIEKIKTI GATYMAASGL NTAQAQDGSH PQEHLQILFE FAKEMMRVVD DFNNNMLWFN FKLRVGFNHG PLTAGVIGTT KLLYDIWGDT VNIASRMDTT GVECRIQVSE ESYRVLSKMG YDFDYRGTVN VKGKGQMKTY LYPKCTDHRV IPQHQLSISP DIRVQVDGSI GRSPTDEIAN LV
Sequence Length
1353
Applicable Applications for ADCY9 recombinant protein
SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Organism
Homo sapiens (Human)
Expression System
Prokaryotic expression
Residues
Asp1029~Val1282 (Accession # O60503) with two N-terminal Tags, His-tag and T7-tag
Subcellular Location
Membrane; Multi-pass membrane protein
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method)
Predicted Isoelectric Point
6
Reconstitution
Reconstitute in sterile PBS, pH7.2-pH7.4.
Preparation and Storage
Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

SDS-Page

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
115
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
32.6kDa
NCBI Official Full Name
adenylate cyclase type 9
NCBI Official Synonym Full Names
adenylate cyclase 9
NCBI Official Symbol
ADCY9
NCBI Official Synonym Symbols
AC9
NCBI Protein Information
adenylate cyclase type 9; adenylyl cyclase 9; ATP pyrophosphate-lyase 9; adenylate cyclase type IX; type IX ATP pyrophosphate-lyase
UniProt Protein Name
Adenylate cyclase type 9
Protein Family
UniProt Gene Name
ADCY9
UniProt Synonym Gene Names
KIAA0520
UniProt Entry Name
ADCY9_HUMAN

NCBI Description

Adenylate cyclase is a membrane bound enzyme that catalyses the formation of cyclic AMP from ATP. It is regulated by a family of G protein-coupled receptors, protein kinases, and calcium. The type 9 adenylyl cyclase is a widely distributed adenylyl cyclase, and it is stimulated by beta-adrenergic receptor activation but is insensitive to forskolin, calcium, and somatostatin. [provided by RefSeq, Jul 2008]

Uniprot Description

ADCY9: May play a fundamental role in situations where fine interplay between intracellular calcium and cAMP determines the cellular function. May be a physiologically relevant docking site for calcineurin. Belongs to the adenylyl cyclase class-4/guanylyl cyclase family.

Protein type: Adenylyl cyclase; Transporter, aquaporin family; EC 4.6.1.1; Lyase; Transporter; Membrane protein, multi-pass; Membrane protein, integral; Nucleotide Metabolism - purine

Chromosomal Location of Human Ortholog: 16p13.3

Cellular Component: integral to plasma membrane; plasma membrane

Molecular Function: metal ion binding; ATP binding; adenylate cyclase activity

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; water transport; activation of protein kinase A; signal transduction; G-protein signaling, adenylate cyclase activating pathway; synaptic transmission; phospholipase C activation; energy reserve metabolic process; renal water homeostasis; innate immune response; cAMP biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; transmembrane transport

Research Articles on ADCY9

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Product Notes

The ADCY9 adcy9 (Catalog #AAA2018364) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Adenylate Cyclase 9 (ADCY9) can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the ADCY9 adcy9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- DW LLRNIIPYHV AEQLKVSQTY SKNHDSGGVI FASIVNFSEF YEENYEGGKE CYRVLNELIG DFDELLSKPD YSSIEKIKTI GATYMAASGL NTAQAQDGSH PQEHLQILFE FAKEMMRVVD DFNNNMLWFN FKLRVGFNHG PLTAGVIGTT KLLYDIWGDT VNIASRMDTT GVECRIQVSE ESYRVLSKMG YDFDYRGTVN VKGKGQMKTY LYPKCTDHRV IPQHQLSISP DIRVQVDGSI GRSPTDEIAN LV. It is sometimes possible for the material contained within the vial of "Adenylate Cyclase 9 (ADCY9), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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