Human ADAMTS-like 4 ELISA Kit | ADAMTSL4 elisa kit

Human ADAMTS-like protein 4, ADAMTSL4 ELISA Kit

Cat. Num. AAA931950

• Gene Names: ADAMTSL4; TSRC1; ECTOL2; ADAMTSL-4
• Reactivity: Human
• Synonyms: ADAMTS-like 4; Human ADAMTS-like protein 4; ADAMTSL4 ELISA Kit; Human ADAMTS-like protein 4 (ADAMTSL4) ELISA kit; TSRC1; OTTHUMP00000032789; thrombospondin repeat containing 1; ADAMTSL4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human ADAMTSL4. No significant cross-reactivity or interference between human ADAMTSL4 and analogues was observed.
• Sequence Length: 1035

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: < 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTSL4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADAMTSL4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADAMTSL4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADAMTSL4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADAMTSL4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 568384851
• NCBI GeneID: 54507
• NCBI Accession #: NP_001275536.1
• NCBI GenBank Nucleotide #: NM_001288607.1
• UniProt Accession #: Q6UY14; Q5T5F7; Q6IPM6; Q8N643; Q9HBS6; B2RTT0; F8WAD0
• Molecular Weight: 116,545 Da
• NCBI Official Full Name: ADAMTS-like protein 4 isoform 3
• NCBI Official Synonym Full Names: ADAMTS-like 4
• NCBI Official Symbol: ADAMTSL4
• NCBI Official Synonym Symbols: TSRC1; ECTOL2; ADAMTSL-4
• NCBI Protein Information: ADAMTS-like protein 4; thrombospondin repeat-containing protein 1
• UniProt Protein Name: ADAMTS-like protein 4
• Protein Family: ADAMTS-like protein
• UniProt Gene Name: ADAMTSL4
• UniProt Synonym Gene Names: TSRC1; PP1396; UNQ2803/PRO34012; ADAMTSL-4
• UniProt Entry Name: ATL4_HUMAN

NCBI Description

This gene is a member of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs)-like gene family and encodes a protein with seven thrombospondin type 1 repeats. The thrombospondin type 1 repeat domain is found in many proteins with diverse biological functions including cellular adhesion, angiogenesis, and patterning of the developing nervous system. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Sep 2014]

Uniprot Description

TSRC1: Positive regulation of apoptosis. May facilitate FBN1 microfibril biogenesis. Defects in ADAMTSL4 are a cause of autosomal recessive isolated ectopia lentis (ARI-EL). ARI-EL is a rare condition characterized by partial or complete displacement of the lens from its space resulting from defective zonule formation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 1q21.3

Cellular Component: endoplasmic reticulum lumen

Molecular Function: protein binding; protease binding; metalloendopeptidase activity

Biological Process: protein amino acid O-linked glycosylation; extracellular matrix organization and biogenesis; cellular protein metabolic process; positive regulation of apoptosis; apoptosis; proteolysis; post-translational protein modification

Disease: Ectopia Lentis 2, Isolated, Autosomal Recessive; Ectopia Lentis Et Pupillae