Human ADAM metallopeptidase with thrombospondin type 1 motif, 13 ELISA Kit | ADAMTS13 elisa kit

Human von Willebrand Factor cleaving protease, ADAMTS-13/vWF-cp ELISA Kit

Cat. Num. AAA705447

• Gene Names: Adamts13; Gm710; vWF-CP; ADAM-TS13; ADAMTS-13
• Reactivity: Human
• Synonyms: ADAM metallopeptidase with thrombospondin type 1 motif; 13; Human von Willebrand Factor cleaving protease; ADAMTS-13/vWF-cp ELISA Kit; C9orf8; DKFZp434C2322; FLJ42993; MGC118899; MGC118900; TTP; VWFCP; vWF-CP; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif; vWF-cleaving protease; von Wil; ADAMTS13 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ADAMTS13. No significant cross-reactivity or interference between Human ADAMTS13 and analogues was observed.
• Sequence Length: 1426

• Samples: Serum, plasma, tissue homogenates, urine, cell culture supernates
• Detection Range: 78 pg/ml-5000 pg/ml
• Sensitivity: 19.5 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Blood Coagulation
• Protein Biological Process 3: Blood coagulation
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAMTS13 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to vWF-cp. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for vWF-cp and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain vWF-cp, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of vWF-cp in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 47604978
• NCBI GeneID: 279028
• NCBI Accession #: NP_001001322.1
• NCBI GenBank Nucleotide #: NM_001001322.2
• UniProt Accession #: Q769J6; Q76LW1; A2ALB4
• Molecular Weight: 155,357 Da
• NCBI Official Full Name: A disintegrin and metalloproteinase with thrombospondin motifs 13 isoform 1
• NCBI Official Synonym Full Names: a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 13
• NCBI Official Symbol: Adamts13
• NCBI Official Synonym Symbols: Gm710; vWF-CP; ADAM-TS13; ADAMTS-13
• NCBI Protein Information: A disintegrin and metalloproteinase with thrombospondin motifs 13; vWF-cleaving protease; von Willebrand factor-cleaving protease; vWF-CP mRNA for von Willebrand factor-cleaving; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 13
• UniProt Protein Name: A disintegrin and metalloproteinase with thrombospondin motifs 13
• Protein Family: A disintegrin and metalloproteinase with thrombospondin motifs
• UniProt Gene Name: Adamts13
• UniProt Synonym Gene Names: Gm710; ADAM-TS 13; ADAM-TS13; ADAMTS-13; vWF-CP; vWF-cleaving protease
• UniProt Entry Name: ATS13_MOUSE

Uniprot Description

Function: Cleaves the vWF multimers in plasma into smaller forms thereby controlling vWF-mediated platelet thrombus formation.

Catalytic activity: Cleaves the vWF at the 842-Tyr-|-Met-843 in the A2 domain of the vWF subunit.

Cofactor: Binds 1 zinc ion per subunit

Enzyme regulation: Zinc and calcium ions cooperatively modulate enzyme activity. The cleavage of the pro-domain is not required for protease activity. Dependence on calcium for proteolytic activity is mediated by the high affinity site