Human ADAM metallopeptidase domain 9 (meltrin gamma) ELISA Kit | ADAM9 elisa kit

Human A Disintegrin And Metalloprotease 9, ADAM9 ELISA Kit

Cat. Num. AAA703902

• Gene Names: ADAM9; MCMP; MDC9; CORD9; Mltng
• Reactivity: Human
• Synonyms: ADAM metallopeptidase domain 9 (meltrin gamma); Human A Disintegrin And Metalloprotease 9; ADAM9 ELISA Kit; CORD9; KIAA0021; MCMP; MDC9; Mltng; ADAM metallopeptidase domain 9; a disintegrin and metalloproteinase domain 9 (meltrin gamma) ; cellular disintegrin-related protein; meltrin gamma; myeloma cell metall; ADAM9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ADAM9. No significant cross-reactivity or interference between Human ADAM9 and analogues was observed.
• Sequence Length: 819

• Samples: Serum, plasma, tissue homogenates, cell culture supernates and ascitic fluid
• Detection Range: 62.5 pg/ml-4000 pg/ml
• Sensitivity: 15.6 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM9 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ADAM9. Standards or samples are then added to the appropriate microtiter plate wells with a HRP-conjugated antibody preparation specific for ADAM9 to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ADAM9, HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ADAM9 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4501915
• NCBI GeneID: 8754
• NCBI Accession #: NP_003807.1
• NCBI GenBank Nucleotide #: NM_003816.2
• UniProt Accession #: Q13443; Q10718; Q8NFM6; B7ZLN7
• Molecular Weight: 90,556 Da
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 9
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 9
• NCBI Official Symbol: ADAM9
• NCBI Official Synonym Symbols: MCMP; MDC9; CORD9; Mltng
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 9; cone rod dystrophy 9; myeloma cell metalloproteinase; cellular disintegrin-related protein; ADAM metallopeptidase domain 9 (meltrin gamma); metalloprotease/disintegrin/cysteine-rich protein 9
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 9
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM9
• UniProt Synonym Gene Names: KIAA0021; MCMP; MDC9; MLTNG; ADAM 9
• UniProt Entry Name: ADAM9_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Function: Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.1 PublicationManual assertion based on experiment in:Ref.3

Cofactor: Binds 1 zinc ion per subunit Inferred.