Mouse A Disintegrin And Metalloprotease 9 ELISA Kit | ADAM9 elisa kit

Mouse A Disintegrin And Metalloprotease 9 ELISA Kit

Cat. Num. AAA728187

• Gene Names: ADAM9; MCMP; MDC9; CORD9; Mltng
• Reactivity: Mouse
• Synonyms: A Disintegrin And Metalloprotease 9; Mouse A Disintegrin And Metalloprotease 9 ELISA Kit; ADAM9 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This ADAM9 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse ADAM9. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ADAM9 elisa kit

Principle of the assay: ADAM9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ADAM9 antibody and an ADAM9-HRP conjugate. The assay sample and buffer are incubated together with ADAM9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM9 concentration since ADAM9 from samples and ADAM9-HRP conjugate compete for the anti-ADAM9 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM9 from the sample, fewer sites are left to bind ADAM9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM9 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ADAM9 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 116496893
• NCBI GeneID: 8754
• Molecular Weight: 72,359 Da
• NCBI Official Full Name: ADAM9 protein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 9
• NCBI Official Symbol: ADAM9
• NCBI Official Synonym Symbols: MCMP; MDC9; CORD9; Mltng
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 9; cone rod dystrophy 9; myeloma cell metalloproteinase; cellular disintegrin-related protein; ADAM metallopeptidase domain 9 (meltrin gamma); metalloprotease/disintegrin/cysteine-rich protein 9
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 9
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM9
• UniProt Synonym Gene Names: KIAA0021; MCMP; MDC9; MLTNG; ADAM 9
• UniProt Entry Name: ADAM9_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. Several alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

ADAM9: Probable zinc protease. May mediate cell-cell or cell- matrix interactions. Isoform 2 displays alpha-secretase activity for APP. Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.-; Protease

Chromosomal Location of Human Ortholog: 8p11.22

Cellular Component: extracellular space; cell surface; focal adhesion; basolateral plasma membrane; cytoplasm; integral to membrane

Molecular Function: integrin binding; collagen binding; protein binding; protein kinase C binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; laminin binding; SH3 domain binding

Biological Process: integrin-mediated signaling pathway; positive regulation of keratinocyte migration; extracellular matrix organization and biogenesis; activation of MAPKK activity; cell-matrix adhesion; response to glucocorticoid stimulus; membrane protein ectodomain proteolysis; positive regulation of cell adhesion mediated by integrin; positive regulation of membrane protein ectodomain proteolysis; response to manganese ion; keratinocyte differentiation; extracellular matrix disassembly; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; response to hydrogen peroxide; monocyte activation; positive regulation of protein secretion; transforming growth factor beta receptor signaling pathway; cell-cell adhesion mediated by integrin; cell adhesion mediated by integrin; response to calcium ion; cell adhesion

Disease: Cone-rod Dystrophy 9