A Disintegrin And Metalloprotease 17 (ADAM17) Recombinant Protein | ADAM17 recombinant protein

Recombinant A Disintegrin And Metalloprotease 17 (ADAM17)

Cat. Num. AAA2011531

• Gene Names: ADAM17; CSVP; TACE; NISBD; ADAM18; CD156B
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: A Disintegrin And Metalloprotease 17 (ADAM17); Recombinant A Disintegrin And Metalloprotease 17 (ADAM17); ADAM17 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-KLLVVAD HRFYRYMGRG EESTTTNYLI ELIDRVDDIY RNTSWDNAGF KGYGIQIEQI RILKSPQEVK PGEKHYNMAK SYPNEEKDAW DVKMLLEQFS FDIAEEASKV CLAHLFTYQD FDMGTLGLAY VGSPRANSHG GVCPKAYYSP VGKKNIYLNS GLTSTKNYGK TILTKEADLV TTHELGHNFG AEHDPDGLAE CAPNEDQGGK YVMYPIAVSG DHENNKMFSN CSKQSIYKTI ESKAQECFQE R
• Sequence Length: 824

• Applicable Applications for ADAM17 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Lys226~Arg473 (Accession # P78536) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 73747889
• NCBI GeneID: 6868
• NCBI Accession #: NP_003174.3
• NCBI GenBank Nucleotide #: NM_003183.4
• UniProt Accession #: P78536; O60226
• Molecular Weight: 29.5kDa
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 17 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 17
• NCBI Official Symbol: ADAM17
• NCBI Official Synonym Symbols: CSVP; TACE; NISBD; ADAM18; CD156B
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 17; TNF-alpha convertase; snake venom-like protease; TNF-alpha converting enzyme; ADAM metallopeptidase domain 18; tumor necrosis factor, alpha, converting enzyme
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 17
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM17
• UniProt Synonym Gene Names: CSVP; TACE; ADAM 17
• UniProt Entry Name: ADA17_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene functions as a tumor necrosis factor-alpha converting enzyme; binds mitotic arrest deficient 2 protein; and also plays a prominent role in the activation of the Notch signaling pathway. [provided by RefSeq, Jul 2008]

Uniprot Description

TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala-|-Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, and the amyloid precursor protein. Also involved in the activation of Notch pathway. Binds 1 zinc ion per subunit. Two splice variant isoforms have been described.

Protein type: EC 3.4.24.86; Motility/polarity/chemotaxis; Protease; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2p25

Cellular Component: focal adhesion; cell surface; membrane; integral to plasma membrane; cytoplasm; apical plasma membrane; plasma membrane; intercellular junction; lipid raft; actin cytoskeleton

Molecular Function: integrin binding; protein binding; interleukin-6 receptor binding; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; Notch binding; SH3 domain binding; PDZ domain binding

Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; neutrophil mediated immunity; T cell differentiation in the thymus; cell motility involved in cell locomotion; positive regulation of leukocyte chemotaxis; response to lipopolysaccharide; proteolysis; G1/S-specific positive regulation of cyclin-dependent protein kinase activity; extracellular matrix disassembly; positive regulation of epidermal growth factor receptor activity; positive regulation of cell proliferation; germinal center formation; negative regulation of interleukin-8 production; cell adhesion; response to drug; spleen development; epidermal growth factor receptor signaling pathway; Notch signaling pathway; response to high density lipoprotein stimulus; membrane protein intracellular domain proteolysis; membrane protein ectodomain proteolysis; positive regulation of transforming growth factor beta receptor signaling pathway; Notch receptor processing; positive regulation of cell motility; positive regulation of cell growth; positive regulation of chemokine production; regulation of mast cell apoptosis; collagen catabolic process; PMA-inducible membrane protein ectodomain proteolysis; B cell differentiation; response to hypoxia; cell adhesion mediated by integrin; positive regulation of protein amino acid phosphorylation; negative regulation of transforming growth factor beta receptor signaling pathway; positive regulation of cell migration; wound healing, spreading of epidermal cells

Disease: Inflammatory Skin And Bowel Disease, Neonatal, 1