Human A Disintegrin And Metalloprotease 10 ELISA Kit | ADAM10 elisa kit
Human A Disintegrin And Metalloprotease 10 ELISA Kit
Principle of the Assay||ADAM10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ADAM10 antibody and an ADAM10-HRP conjugate. The assay sample and buffer are incubated together with ADAM10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAM10 concentration since ADAM10 from samples and ADAM10-HRP conjugate compete for the anti-ADAM10 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAM10 from the sample, fewer sites are left to bind ADAM10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAM10 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Members of the ADAM family are cell surface proteins with a unique structure possessing both potential adhesion and protease domains. This gene encodes and ADAM family member that cleaves many proteins including TNF-alpha and E-cadherin. [provided by RefSeq, Jul 2008]
Uniprot Description
ADAM10: Cleaves the membrane-bound precursor of TNF-alpha at '76-Ala-|-Val-77' to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including heparin-binding epidermal growth- like factor, ephrin-A2 and for constitutive and regulated alpha- secretase cleavage of amyloid precursor protein (APP). Contributes to the normal cleavage of the cellular prion protein. Involved in the cleavage of the adhesion molecule L1 at the cell surface and in released membrane vesicles, suggesting a vesicle-based protease activity. Controls also the proteolytic processing of Notch and mediates lateral inhibition during neurogenesis. Responsible for the FasL ectodomain shedding and for the generation of the remnant ADAM10-processed FasL (FasL APL) transmembrane form. Also cleaves the ectodomain of the integral membrane proteins CORIN and ITM2B. May regulate the EFNA5-EPHA3 signaling.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; EC 3.4.24.81; Protease; Vesicle; Cell surface; Cell adhesion
Chromosomal Location of Human Ortholog: 15q22
Cellular Component: Golgi apparatus; focal adhesion; cell surface; membrane; intracellular membrane-bound organelle; cytoplasm; postsynaptic density; integral to membrane; plasma membrane; Golgi-associated vesicle; nucleus
Molecular Function: integrin binding; protein binding; protein homodimerization activity; metallopeptidase activity; zinc ion binding; metalloendopeptidase activity; endopeptidase activity; SH3 domain binding; protein kinase binding; receptor binding
Biological Process: integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; axon guidance; extracellular matrix organization and biogenesis; Notch signaling pathway; in utero embryonic development; constitutive protein ectodomain proteolysis; membrane protein ectodomain proteolysis; Notch receptor processing; negative regulation of cell adhesion; positive regulation of cell growth; protein amino acid phosphorylation; collagen catabolic process; extracellular matrix disassembly; PMA-inducible membrane protein ectodomain proteolysis; cell-cell signaling; monocyte activation; positive regulation of cell proliferation; ephrin receptor signaling pathway; positive regulation of cell migration
Disease: Reticulate Acropigmentation Of Kitamura; Alzheimer Disease 18
Research Articles on ADAM10
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Product Notes
The Human ADAM10 adam10 (Catalog #AAA726961) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA726961 ELISA Kit recognizes Human ADAM10. It is sometimes possible for the material contained within the vial of "A Disintegrin And Metalloprotease 10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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