Human Adenosine deaminase-like protein ELISA Kit | ADAL elisa kit

Human Adenosine deaminase-like protein ELISA Kit

Cat. Num. AAA761110

• Reactivity: Human
• Synonyms: Adenosine deaminase-like protein; Human Adenosine deaminase-like protein ELISA Kit; ADAL; ADAL elisa kit

• Reactivity: Human
• Sequence Length: 328

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Competitive ELISA, Coated with Antigen
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ADAL elisa kit

Principle of the Assay||This ELISA kit uses Competitive-ELISA as the method. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ADAL. During the reaction,Human ADAL in the sample or standard competes with a fixed amount of biotin-labeled Human ADAL for sites on a pre-coated Monoclonal antibody specific to Human ADAL. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Human ADAL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 226693318
• NCBI GeneID: 161823
• NCBI Accession #: NP_001152752.1
• NCBI GenBank Nucleotide #: NM_001159280.2
• Molecular Weight: 37,362 Da
• NCBI Official Full Name: adenosine deaminase-like protein isoform 1
• NCBI Official Synonym Full Names: adenosine deaminase-like
• NCBI Official Symbol: ADAL
• NCBI Protein Information: adenosine deaminase-like protein
• UniProt Protein Name: Adenosine deaminase-like protein
• Protein Family: Adenosine deaminase-like protein
• UniProt Gene Name: ADAL
• UniProt Entry Name: ADAL_HUMAN

Uniprot Description

ADAL: Putative nucleoside deaminase. May catalyze the hydrolytic deamination of adenosine or some similar substrate and play a role in purine metabolism. Belongs to the adenosine and AMP deaminases family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Hydrolase; EC 3.5.4.-

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: cytosol

Molecular Function: adenosine deaminase activity; metal ion binding

Biological Process: adenosine catabolic process; drug metabolic process; hypoxanthine salvage; inosine biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; nucleotide metabolic process; purine base metabolic process; purine salvage