Testing Data
(DRG sections double-labeled for A0576-03 (red) and either MBS613826 (green, A) or C1078-25 (green, B); double-labeled cells are yellow or orange)
Rabbit anti-Human, Rat P2X3 Receptor Polyclonal Antibody | anti-P2RX3 antibody
P2X3 Receptor
Serum
Serum
Dilution: Western Blot (Reducing): 1:1000. Cell membrane extracts were examined by SDS-PAGE under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 4 C with PBS, 0.1% Tween 20, 2.5% milk powder. Membranes were incubated with diluted P1003-08B overnight at 4 C then rinsed with PBS and incubated with IgG (HRP) Goat anti-rat for 1 hour at RT. Following rinsing, the membranes were processed using ECL
Immunohistochemistry: 1:1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution. 2) Paraformaldehyde-picric acid fixative. 3) PBS, 10% sucrose as a cryo-protectant. Desired tissues were dissected and stored overnight in PBS, 10% sucrose. Slide-mounted tissue sections were processed for Indirect Immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. P1003-08B was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed. Slides were incubated for 18-24 hours at 4 degree C with primary antiserum. Slides were rinsed 3 times with PBS. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times with PBS and coverslipped. Staining was examined using Fluorescence Microscopy.
Immunocytochemistry: 1:1000. P2X3 transfected cells were processed for Indirect Immunofluorescence. Media was removed. Cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for Indirect Immunofluorescence as described for Immunohistochemistry.
Testing Data
(DRG sections double-labeled for A0576-03 (red) and either MBS613826 (green, A) or C1078-25 (green, B); double-labeled cells are yellow or orange)
Testing Data
(DRG sections double-labeled for A0576-03 (red) and either MBS613826 (green, A) or C1078-25 (green, B); double-labeled cells are yellow or orange)
NCBI and Uniprot Product Information
NCBI Description
The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage. [provided by RefSeq, Jul 2008]
Uniprot Description
P2X3: Receptor for ATP that acts as a ligand-gated ion channel. Belongs to the P2X receptor family.
Protein type: Channel, ligand-gated; Membrane protein, integral; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 11q12
Cellular Component: Golgi apparatus; rough endoplasmic reticulum; cell soma; integral to plasma membrane; dendritic spine; terminal button; integral to nuclear inner membrane; receptor complex; lipid raft
Molecular Function: purinergic nucleotide receptor activity; ATP binding; ATP-gated cation channel activity
Biological Process: urinary bladder smooth muscle contraction; peristalsis; sensory perception of taste; generation of action potential; regulation of synaptic plasticity; positive regulation of calcium-mediated signaling; signal transduction; response to ATP; response to carbohydrate stimulus; behavioral response to pain; response to heat; response to mechanical stimulus; transport; response to hypoxia; neuromuscular synaptic transmission; response to cold; protein homooligomerization
Research Articles on P2RX3
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Product Notes
The P2RX3 p2rx3 (Catalog #AAA613826) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The P2X3 Receptor reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's P2X3 Receptor can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot (Reducing): 1:1000. Cell membrane extracts were examined by SDS-PAGE under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 4 C with PBS, 0.1% Tween 20, 2.5% milk powder. Membranes were incubated with diluted P1003-08B overnight at 4 C then rinsed with PBS and incubated with IgG (HRP) Goat anti-rat for 1 hour at RT. Following rinsing, the membranes were processed using ECL Immunohistochemistry: 1:1000. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution. 2) Paraformaldehyde-picric acid fixative. 3) PBS, 10% sucrose as a cryo-protectant. Desired tissues were dissected and stored overnight in PBS, 10% sucrose. Slide-mounted tissue sections were processed for Indirect Immunofluorescence. Slides were incubated with blocking buffer for 1 hour at RT. P1003-08B was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed. Slides were incubated for 18-24 hours at 4 degree C with primary antiserum. Slides were rinsed 3 times with PBS. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times with PBS and coverslipped. Staining was examined using Fluorescence Microscopy. Immunocytochemistry: 1:1000. P2X3 transfected cells were processed for Indirect Immunofluorescence. Media was removed. Cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for Indirect Immunofluorescence as described for Immunohistochemistry. Researchers should empirically determine the suitability of the P2RX3 p2rx3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "P2X3 Receptor, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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