Vitronectin Active Protein | VTN active protein

Vitronectin, Recombinant, Human

Cat. Num. AAA638112

• Gene Names: VTN; VN; V75; VNT
• Applications: ELISA
• Purity: Highly Purified; ~90% as determined by SDS-PAGE and visualized by silver stain.
• Synonyms: Vitronectin; Recombinant; Human; VTN active protein

• Purity/Purification: Highly Purified; ~90% as determined by SDS-PAGE and visualized by silver stain.
• Form/Format: Supplied as a lyophilized powder from 50mM Tris, 0.15M sodium chloride, pH 8.0. Reconstitute with 500ul sterile PBS, 0.1% HSA or BSA.

• Applicable Applications for VTN active protein: ELISA (EL/EIA)
• Activity: Measured by the ability of the immobilized protein to support the adhesion of DU145 cells. When 5x10e4 cells/well are added to Vitronectin coated plates (5ug/ml and 100ul/well), approximately 55-75% will adhere after 30 minutes at 37 degree C.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months. Reconstitute with sterile PBS, 01% BSA or HSA. Aliquot and store at -20 degree C/-70 degree C. Reconstituted product is stable for 6 months at --20 degree C/-70 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for VTN active protein

Vitronectin is a large glycoprotein found in blood and the extracellular matrix (ECM). The gene for vitronectin encodes a 19 amino acid (aa) signal peptide and a 459 aa protein. The amino terminal 130 aa residues of vitronectin contains multiple binding sites for a variety of structures. Included is a site for binding to plasminogen activator inhibitor-1 (PAI-1) and urokinase receptor, an (RGD) sequence that binds avb3, avb5, aavb1, aIIbb3, avb6, and avb8 integrins, a stretch of acidic amino acids that includes two sulfated tyrosine residues that bind thrombin-anti-thrombin III complexes, and a collagen binding site. The major part of the vitronectin molecule (aa 132-459) contains six hemopexin-like repeats. The carboxyl-terminal end of vitronectin also has multiple sites and functions. It contains a stretch of basic amino acids that binds the acidic amino acids of the amino-terminal region, thereby stabilizing vitronectin's three dimensional structure. The carboxyl-terminal end also contains a plaminogen binding site, a heparin binding site that binds complement factor C7, C8 or C9, a glycosaminoglycan binding site, and a second PAI-1 binding site (aa 348-370). Vitronectin also contains an endogenous cleavage site, plus cleavage sites for elastase, thrombin and plasmin. Vitronectin has also been shown to bind IGF-2 and TGF-b.

The apparent molecular weight of human vitronectin is 75 kDa, with ~30% of its molecular mass being attributed to glycosylation at 3 different sites. In blood and plasma, vitronectin is found predominantly as a single chain monomer. It can also be found as a dimer after endogenous cleavage. The dimer is composed of a 65kD and 10kD component held together by a disulfide bond. Binding of thrombin-anti-thrombin II complex or complement leads to an unfolding of vitronectin. Unfolding of vitronectin generates disulfide-linked multimers that are found in platelet secretions and extracellular matrix. Vitronectin is produced at high levels by the liver and many tumors. As might be expected by its structure, Vitronectin is involved in a number of biological activities including cell adhesion, cell spreading and migration, cell proliferation, extracellular anchoring, fibrinolysis, hemostasis, and complement mediated immune defense.

Product Categories/Family for VTN active protein

Molecular Biology; MB-Vitronectin

NCBI and Uniprot Product Information

• NCBI GI #: 88853069
• NCBI GeneID: 7448
• NCBI Accession #: NP_000629.3
• NCBI GenBank Nucleotide #: NM_000638.3
• UniProt Accession #: P04004; P01141; Q9BSH7; B2R7G0
• Molecular Weight: 52.3kD
• NCBI Official Full Name: vitronectin
• NCBI Official Synonym Full Names: vitronectin
• NCBI Official Symbol: VTN
• NCBI Official Synonym Symbols: VN; V75; VNT
• NCBI Protein Information: vitronectin; epibolin; S-protein; somatomedin B; complement S-protein; serum spreading factor; serum-spreading factor
• UniProt Protein Name: Vitronectin
• Protein Family: Vitronectin
• UniProt Gene Name: VTN
• UniProt Synonym Gene Names: VN
• UniProt Entry Name: VTNC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the pexin family. It is found in serum and tissues and promotes cell adhesion and spreading, inhibits the membrane-damaging effect of the terminal cytolytic complement pathway, and binds to several serpin serine protease inhibitors. It is a secreted protein and exists in either a single chain form or a clipped, two chain form held together by a disulfide bond. [provided by RefSeq, Jul 2008]

Uniprot Description

VTN: Vitronectin is a cell adhesion and spreading factor found in serum and tissues. Vitronectin interact with glycosaminoglycans and proteoglycans. Is recognized by certain members of the integrin family and serves as a cell-to-substrate adhesion molecule. Inhibitor of the membrane-damaging effect of the terminal cytolytic complement pathway.

Protein type: Secreted; Cell adhesion; Secreted, signal peptide; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 17q11

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region

Molecular Function: heparin binding; integrin binding; protein binding; extracellular matrix binding; scavenger receptor activity; polysaccharide binding

Biological Process: receptor-mediated endocytosis; extracellular matrix organization and biogenesis; positive regulation of protein binding; cell-matrix adhesion; negative regulation of blood coagulation; positive regulation of smooth muscle cell migration; positive regulation of receptor-mediated endocytosis; positive regulation of vascular endothelial growth factor receptor signaling pathway; positive regulation of peptidyl-tyrosine phosphorylation; regulation of complement activation; innate immune response; cell adhesion mediated by integrin; immune response; cell adhesion